离子交换树脂吸附古龙酸的工艺

应用自行合成的阴离子树脂D02直接吸附发酵液中的2-酮基-L-古龙酸,并研究了其吸附的静态与动态过程.利用Langmuir和Freundlich吸附等温线方程对吸附等温线数据进行拟合,发现2种模型相关性都很好.测定了不同流速、温度、浓度以及pH条件下古龙酸在合成树脂柱中的穿透曲线,当上柱液pH为1.5、质量浓度为10 g/L、吸附温度为309 K、流速为0.35 mL/min时,每g湿树脂的动态附容量为133 mg.同时测定了不同洗脱剂、洗脱流速条件下的洗脱曲线.每g湿树脂的动态吸附容量达到了133 mg,当温度为283 K、洗脱剂为10%H2SO4-CH3OH、流速为0.2 mL/min时,洗脱收率可达65.3%.

Adsorptive separation process of KGA onto D02 adsorption resin

The adsorptive separation processes and model of 2-keto-L-gulonic acid in aqueous solution by resin D02 had been investigated.Equilibrium data for the adsorption of KGA from aqueous solutions with resin D02 had been researched,which is correlated with a langmuir-type and Freundlich-type of isotherm equations.The adsorption capability was 133 mg KGA/g wet resin when the concertration of KGA was 10 g/L,the temperature was 309 K,the adsorption velocity was 0.35 mL/min.The experiments of both break thought and elution curves of 2-keto-L-gulonic acid in the filled with resin D02 were carried out at 298.15, 308.15 and 318.15 K.The effects of flow rates,feed concentrations and pH values on the break thought curve were evaluated.The elution rate of KGA was 3.5% when the eluent was 10% H2SO4-CH3OH,the temperature was 283 K and the flow rate of the elution was 0.2 mL/min.