载酶海藻酸钙复合微球稳定水包油型Pickering乳液及其强化界面酶催化反应

以疏水改性二氧化钛修饰的载酶海藻酸钙微球（E@Alg@s-TiO₂微球）稳定水包油（O/W）型Pickering乳液用于两相界面酶催化反应。与传统的两相游离酶体系相比，此体系以绿色、温和的方式将酶固定在乳液界面上，并强化了两相界面酶催化反应。研究成果归纳如下：油水比为1∶1.2时，Pickering乳液为O/W型；E@Alg@s-TiO₂微球浓度为3%（质量）时稳定效果最好，脂肪酶的负载量为15.8 mg·g^-1。以三丁酸甘油酯的水解反应为研究对象，该体系对油水体系的界面酶催化反应有很好的强化效果，具有96%转化率并提高酶活力7.8倍。重复使用5个批次能保留80%的酶活力。本研究进一步拓展了载酶海藻酸盐微球稳定的Pickering乳液体系的应用范围，有望为O/W体系的界面生物催化过程提供绿色平台。

E@Alg@s-TiO2 microsphere stabilized O/W Pickering emulsion and the enhancement of interfacial enzymatic catalysis

Hydrophobically-modified titanium dioxide-modified calcium alginate microspheres (E@Alg@s-TiO₂ microspheres) stabilized oil-in-water (O/W) type Pickering emulsion is used for two-phase interfacial enzyme catalysis. Compared with the traditional two-phase free enzyme system, the system immobilizes the enzyme at the emulsion interface in a green and gentle manner, and strengthens the two-phase interface enzyme catalyzed reaction. The research results are summarized as follows: when the oil-water ratio is 1∶1.2, the O/W emulsion is obtained; the concentration of the microspheres is 3%(mass). The O/W system has a good enhancement effect on the biphasic enzyme-catalyzed hydrolysis reaction, with 96% conversion rate and 7.8 times higher enzyme activity than the free enzyme in conventional two-phase system, 5 batches cycles with 80% of residue enzyme activity. This study further expands the application range of the enzyme-loaded alginate microsphere-stabled Pickering emulsion system, which is expected to provide a green platform for the interfacial biocatalysis process.