酶催化合成高分子量聚丁二酸丁二醇酯(PBS)及其材料特性

建立了以固定化南极假丝酵母脂肪酶B(Novozym-435)作为催化剂,以丁二酸二乙酯和1,4-丁二醇作为原料,酶催化合成聚丁二酸丁二醇酯(PBS)的工艺。在95℃,真空条件下经过25 h反应,得到重均分子量44000的PBS,分子量分布为1.64。通过红外光谱、核磁共振、元素分析等分析了合成PBS的组成及分子结构。对酶催化聚合反应过程的动力学,以及反应过程中固定化酶的热稳定性进行了研究。通过对PBS材料性能的表征,证明其具有良好的耐热性能、力学性能和生物降解性能。

Lipase-catalyzed synthesis and characterization of high-molecular-weight PBS

In the present study, high-molecular-weight PBS was synthesized by using immobilized Candida antarctica lipase B(Novozym-435)as catalyst and diethyl succinate and 1,4-butanediol as reagents.The PBS with molecular weight of 44000 was synthesized after 25 h polymerization at 95℃ and 10 mmHg vacuum, which was the highest molecular weight of PBS synthesized by the enzymatic method reported so far.The structure of polyester was determined by IR, NMR and elemental analysis.Enzymatic kinetics of PBS synthesis was monitored and the stability of lipase was investigated.In addition, thermal stability and biodegradable ability of blended polymer materials of enzyme-catalyzed polyester and chemical-catalyzed polyester were also investigated, displaying wonderful performance of synthesized PBS in the field of food and medical application.