异养小球藻产总脂肪酸的培养基优化

研究了异养小球藻(Chlorella protothecoides)分批培养基中葡萄糖和硝酸盐浓度对生物量、总脂和总脂肪酸产量的影响.结果表明,当细胞在含有40 g·L-1葡萄糖和0.1 mol·L-1 NaNO3的异养培养基中生长时,总脂和总脂肪酸的产量可分别达到最高值为3.83 g·L-1和1.64 g·L-1,同时C18脂肪酸占总脂肪酸的比例超过69%,总脂肪酸占总脂的比例也高达42.63%,总脂含量约为24.3%.通过优化异养小球藻培养基中的葡萄糖和硝酸盐浓度,能够获得高产总脂肪酸,进而生产生物柴油.

Optimization of Culture Medium for Heterotrophic Chlorella Protothecoids Producing Total Fatty Acids

The effects of glucose and nitrate concentrations in the batch culture medium on the yields of biomass, total lipids and total fatty acids of heterotrophic Chlorella protothecoides were investigated. The results showed the maximal yields of total lipids(3.83 g·L-1) and total fatty acids(1.64 g·L-1) were achieved in the cells growing in heterotrophic medium containing 40 g·L-1 glucose and 0.1 mol·L-1 NaNO3. Meanwhile, C18 fatty acids was over 69% of total fatty acids as well as a high ratio of total fatty acids to total lipids(42.63%) were observed, with a lipid content of approximately 24.3%. It indicated that high yield of total fatty acids from heterotrophic C. protothecoides could be achieved by optimizing glucose and nitrate concentrations in the medium for further biodiesel production.