| Rhodococcus sp. SHZ-1腈水合酶的诱导表达和稳定性研究 |
| |
| 引用本文： | 王超,张根林,徐小琳,李春.Rhodococcus sp. SHZ-1腈水合酶的诱导表达和稳定性研究[J].中国化学工程学报,2007,15(4):573-578. |
| |
| 作者姓名： | 王超 张根林 徐小琳 李春 |
| |
| 作者单位： | [1]Department of Environmental and Biochemical Engineering, School of Chemistry and Chemical Engineering,Shihezi University, Shihezi 832003, China [2]School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China |
| |
| 基金项目： | 国家自然科学基金，教育部新世纪优秀人才支持计划，the Key Research Projects in the Uygur Autonomous Region of Xinjiang |
| |
| 摘 要： | Inducing expression and the reaction characteristic of nitrile hydratase (NHase) from Rhodococcus sp.SHZ-1 were investigated. The results showed that the expression of NHase was greatly enhanced with the cooperation of acrylonitrile and ammonium chloride as inducer in the medium and the specific activity of NHase was increased of 44%. Then the temperature, pH, concentration of acrylonitrile and acrylamide were evaluated, which affected the activity and reaction characteristic of NHase. It was found that the temperature and concentration of acrylamide were the most important factors for the catalyzation of NHase. The optimal catalysis temperature of NHase from Rhodococcus sp. SHZ-1 was 30℃, and the activation energy of the hydration of NHase was 90.2kJ·mol-1 in the temperature range from 5℃ to 30℃. Km of NHase was 0.095mol.L-1 using acrylonitrile(AN)as substrate, and NHase activity was inhibited seriously when acrylonitrile concentration was up to 40g·L-1, the substrate inhibition constant Ki is 0.283mol·L-1. Moreover, the NHase from Rhodococcus sp. SHZ-1 had very strong tolerance to acrylamide, in which the final concentration of acrylamide reached to 642g·L-1 and the residual activity of NHase still maintained 8.6% of the initial enzyme activity.
|
| 关 键 词： | .腈水合酶 生物触媒 丙烯酰胺 特性 高酶活发酵工艺 |
| 收稿时间： | 26 March 2006 |
| 修稿时间： | 2006-03-26 |
Inducing expression and reaction characteristic of nitrile hydratase from Rhodococcus sp. SHZ-1 |
| |
| WANG Chao,ZHANG Genlin,XU Xiaolin,LI Chun.Inducing expression and reaction characteristic of nitrile hydratase from Rhodococcus sp. SHZ-1[J].Chinese Journal of Chemical Engineering,2007,15(4):573-578. |
| |
| Authors: | WANG Chao ZHANG Genlin XU Xiaolin LI Chun |
| |
| Affiliation: | Department of Environmental and Biochemical Engineering, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China b School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China |
| |
| Abstract: | Inducing expression and the reaction characteristic of nitrile hydratase (NHase) from |
| |
| Keywords: | nitrile hydratase biocatalysis acrylamide characteristic Rhodococcus sp SHZ-1 |
| 本文献已被 CNKI 维普 万方数据 ScienceDirect 等数据库收录！ |
| 点击此处可从《中国化学工程学报》浏览原始摘要信息 |
|
点击此处可从《中国化学工程学报》下载全文 |
|
