| 高效液相法色谱法测定猪殃殃中绿原酸含量 |
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| 引用本文： | 陈剑,刘频健,李洁琼.高效液相法色谱法测定猪殃殃中绿原酸含量[J].广州化工,2014(24):116-117,161. |
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| 作者姓名： | 陈剑 刘频健 李洁琼 |
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| 作者单位： | 九江学院基础医学院,江西 九江,332000 |
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| 摘 要： | 建立了猪殃殃中绿原酸含量的HPLC测定方法。色谱柱为Diamonsil C185μm（250 mm×4.6 mm）色谱柱；流动相为甲醇-0.04％磷酸（23：77）；检测波长为324 nm；流速为1 mL/min；进样量为20μL；柱温25℃。经方法学考察，该方法线性关系、精密度、重现性和稳定性好。可快速、准确用于猪殃殃中绿原酸的含量测定。
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| 关 键 词： | 猪殃殃 绿原酸 高效液相色谱法 |
Determination of Chlorogenic Acid in Cleaver by HPLC |
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| CHEN Jian
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LIU Pin-jian
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LI Jie-qiong.Determination of Chlorogenic Acid in Cleaver by HPLC[J].GuangZhou Chemical Industry and Technology,2014(24):116-117,161. |
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| Authors: | CHEN Jian LIU Pin-jian LI Jie-qiong |
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| Affiliation: | CHEN Jian;LIU Pin-jian;LI Jie-qiong;School of Medicine,Jiujiang University; |
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| Abstract: | The HPLC method for determination the content of chlorogenic acid in cleavers was established. The column was Diamonsil C18 5 μm （250 mm×4. 6 mm）, the mobile phase was methanol -0. 04%phosphoric acid （23：77 ） , the detection wavelength was 324 nm, the flow rate was 1 mL/min, and the column temperature was 25℃. Through the methodology study, it had the characteristics of the linear relation of the method, precision, reproducibility and stability, which can be used identified Cleavers chlorogenic acid quickly and accurately. |
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| Keywords: | Cleaver chlorogenic acid HPLC |
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