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响应面优化淫羊藿抗氧化多酚超声提取工艺
引用本文:毛白杨,郑洁,赵晓红,何陈丹,徐秀泉.响应面优化淫羊藿抗氧化多酚超声提取工艺[J].广州化工,2014(23):64-66.
作者姓名:毛白杨  郑洁  赵晓红  何陈丹  徐秀泉
作者单位:1. 亚邦医药研究所有限公司,江苏 常州,213163
2. 江苏大学京江学院,江苏 镇江,212013
3. 江苏大学药学院,江苏 镇江,212013
基金项目:江苏省中医药管理局项目(LZ13244);江苏大学大学生科研立项项目(12C210)。
摘    要:以总多酚得率和DPPH自由基清除率为指标,采用响应面设计法优化淫羊藿抗氧化活性多酚超声提取工艺。结果表明以多酚得率为指标时,最佳工艺条件为:57%乙醇,料液比28 mL/g,在50℃下超声提取25 min,总多酚得率为34.58 mg/g;以DPPH自由基清除率为指标时,最佳工艺条件为:58%乙醇,料液比30 mL/g,在50℃下超声提取22 min, DPPH自由基清除率为88.4%。

关 键 词:淫羊藿  抗氧化活性多酚  超声提取  响应面

Optimization of Ultrasonic-assisted Extraction of Antioxidant Polyphenols from Epimedii Folium by Response Surface Methodology
MAO Bai-yang,ZHENG Jie,ZHAO Xiao-hong,HE Chen-dan,XU Xiu-quan.Optimization of Ultrasonic-assisted Extraction of Antioxidant Polyphenols from Epimedii Folium by Response Surface Methodology[J].GuangZhou Chemical Industry and Technology,2014(23):64-66.
Authors:MAO Bai-yang  ZHENG Jie  ZHAO Xiao-hong  HE Chen-dan  XU Xiu-quan
Affiliation:MAO Bai-yang;ZHENG Jie;ZHAO Xiao-hong;HE Chen-dan;XU Xiu-quan;Yabang Pharmaceutical & Chemical Co.,Ltd.;College of Jingjiang,Jiangsu University;College of Pharmacy,Jiangsu University;
Abstract:The ultrasonic-assisted extraction processes of antioxidant polyphenols from Epimedii Folium was optimized by response surface methodology with the extraction rate of polyphenols and scavenging activity of DPPH as response value.The result showed that the optimal extraction conditions were ethanol concentration 57%, liquid-material ratio of 28 mL/g, extraction temperature 50℃, and extraction time 25 min for total polyphenols (34.58 mg/g) , ethanol concentration 58%, liquid-material ratio of 30 mL/g, extraction temperature 50 ℃, and extraction time 22 min for scavenging activity on DPPH free radical(88.4%).
Keywords:Epimedii Folium  antioxidant polyphenols  ultrasonic-assisted extraction  response surface methodology
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