| 一步提取-HPLC检测化妆品中氟喹诺酮类抗生素研究 |
| |
| 引用本文： | 徐万帮,张莉,刘亚雄,蒋忠军.一步提取-HPLC检测化妆品中氟喹诺酮类抗生素研究[J].广州化工,2012,40(7):128-129,135. |
| |
| 作者姓名： | 徐万帮 张莉 刘亚雄 蒋忠军 |
| |
| 作者单位： | 广东省药品检验所,广东广州,510180 |
| |
| 摘 要： | 采用pH=6.0的NaAc-HAc缓冲液直接提取化妆品中氟喹诺酮类(氧氟沙星、诺氟沙星和环丙沙星)抗生素,建立反相高效液相色谱同时测定化妆品中氧氟沙星、诺氟沙星和环丙沙星的方法。以十八烷基硅烷键合硅胶为填充剂,以0.05 mol/L柠檬酸与1.0 mol/L醋酸铵(77∶1,用三乙胺调节pH至4.0)混合液——乙腈(85∶15)为流动相,检测波长为280 nm的条件下进行测定。结果表明氧氟沙星、诺氟沙星和环丙沙星的响应线性良好,回收率高。
|
| 关 键 词： | 直接提取 氟喹诺酮 化妆品 |
Determination and Extracted Directly of Fluoroqronolone in Cosmetics by HPLC |
| |
| XU Wan-bang,ZHANG Li,LIU Ya-xiong,JIANG Zhong-jun.Determination and Extracted Directly of Fluoroqronolone in Cosmetics by HPLC[J].GuangZhou Chemical Industry and Technology,2012,40(7):128-129,135. |
| |
| Authors: | XU Wan-bang ZHANG Li LIU Ya-xiong JIANG Zhong-jun |
| |
| Affiliation: | (Guangdong Institute of Drug Control,Guangdong Guangzhou 510180,China) |
| |
| Abstract: | A HPLC method was established for the assay of fluoroqronolone antibiotics（ofloxacin,norfloxacin and ciprofloxacin）,which were extracted directly with the buffer of NaAc-HAc（pH 6.0）in cosmetics.The sample was analyzed on C18 with the buffer-acetonitrile（85：15）,which was consisted of 0.05 mol/L citric acid-acetic ammonium（77：1） and adjusting to pH 4.0 with triethanolamine,as the mobile phase and 280 nm as the detection wavelength.This method was proved to be highly effective and practicable. |
| |
| Keywords: | extracted directly fluoroqronolone cosmetics |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
