| 大孔载体固定化脂肪酶 |
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| 引用本文： | 蔡宏举,付大雁,王满意,周鑫,谭天伟.大孔载体固定化脂肪酶[J].过程工程学报,2007,7(4):773-777. |
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| 作者姓名： | 蔡宏举 付大雁 王满意 周鑫 谭天伟 |
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| 作者单位： | 北京化工大学生命科学与技术学院 |
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| 基金项目： | 国家自然科学基金
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国家自然科学基金
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北京市科技计划 |
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| 摘 要： | 用自制大孔载体固定化脂肪酶，对固定化条件进行了优化，比较了固定化酶与游离酶的酶学参数. 结果表明，酶粉与载体质量比为1:1、固定化温度在20~25℃之间、固定化时间1.5 h的条件下，所得固定化酶的酶活最高. 固定化酶的最适pH为8.5，最适温度为40℃，其热稳定性、操作稳定性都比游离酶高，4℃下保存7 d后，酶活仍剩余94%.
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| 关 键 词： | 大孔载体 脂肪酶 固定化酶 |
| 文章编号： | 1009-606X（2007）04-0773-05 |
| 修稿时间： | 2006-11-02 |
Investigation of Immobilized Lipase onto Macroporous Carrier |
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| CAI Hong-ju,FU Da-yan,WANG Man-yi,ZHOU Xin,TAN Tian-wei.Investigation of Immobilized Lipase onto Macroporous Carrier[J].Chinese Journal of Process Engineering,2007,7(4):773-777. |
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| Authors: | CAI Hong-ju FU Da-yan WANG Man-yi ZHOU Xin TAN Tian-wei |
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| Affiliation: | College of Life Science and Technology，Beijing University of Chemical Technology |
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| Abstract: | Immobilization of lipase on macroporous poly(glycidyl methacrylate-co-divinylbenzene) was investigated and followed by the optimization of immobilizing conditions. The optimized results were achieved with the 1:1 mass ratio of lipase to the carrier, coupling temperature between 20~25℃, and immobilizing time of 1.5 h. The optimum pH and temperature of immobilized lipase were 8.5 and 40℃, respectively. The activity of immobilized lipase remained 94% of the origin after storage at 4℃ for 7 d. The experimental results showed that the thermal stability and operational stability were improved in comparison with free lipase. |
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| Keywords: | macroporous carrier lipase immobilized lipase |
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