流行性乙型脑炎减毒活疫苗对国内外不同野毒株的免疫性

目的　研究乙型脑炎减毒活疫苗对国内外不同野毒株的免疫原性。方法　用小鼠保护力试验和空斑减少中和试验检测该疫苗对国内外不同野毒株攻击的保护作用及其免疫血清的中和能力。结果　小鼠免疫 1针后均能抵抗国内 11株和国外 11株乙脑病毒的攻击 ,保护率均可达 90 %以上 ;人体免疫血清对台湾 1株和国外 9株病毒均有明显的中和作用。结论　SA14 142株乙脑活疫苗具有广谱的免疫原性。

Immunity of Live Attenuated Japanese Encephalitis(JE)Vaccine to Different Wild JE Virus Strains

Objective To study on the immunity of live attenuated Japanese Encephalitis(JE)vaccine to different wild JE virus strains.Methods The neutralizing effect of the vaccine on different wild JE virus strains was detected by plaque reduction neutralization test(PRNT),and the immunogenicity of it was studied in mice by vaccination challenge protection test.Results The vaccine could protect mice against the challenge with 11 JE virus strains isolated in China and 11 in Thailand,Vietnam,Indonesia,India,Philippines and Japan.The protective rate of it reached above 90%.Human immune sera showed significant neutralizing effect on 1 JE virus strain isolated in Taiwan and 9 strains isolated abroad.Conclusion Live attenuated JE vaccine prepared with strain SA14 14 2 has broad spectrum immunogenicity.