| 循环应用低剂量环磷酰胺对黑色素瘤荷瘤小鼠调节性T细胞的影响及抗瘤作用 |
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| 引用本文： | 李卫泊,尹昱,蔡建辉,张峻岭,谢绍建,薛平,周保军,严丽.循环应用低剂量环磷酰胺对黑色素瘤荷瘤小鼠调节性T细胞的影响及抗瘤作用[J].粉末涂料与涂装,2010,23(4). |
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| 作者姓名： | 李卫泊 尹昱 蔡建辉 张峻岭 谢绍建 薛平 周保军 严丽 |
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| 作者单位： | 李卫泊,蔡建辉,张峻岭,谢绍建,薛平,周保军,严丽(河北医科大学第二医院,石家庄,050051);尹昱(河北省人民医院康复科,石家庄,050051) |
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| 基金项目： | 河北省科技厅博士基金资助项目，河北省医学适用技术跟踪项目 |
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| 摘 要： | 目的观察循环应用低剂量环磷酰胺(CTX)对黑色素瘤荷瘤小鼠调节性T细胞(Treg)的影响及抗瘤作用。方法通过皮下接种黑色素瘤细胞B16制备黑色素瘤荷瘤小鼠模型;对荷瘤小鼠分别经腹腔单次或循环注射CTX,每隔7d给药1次,共3次,应用流式细胞术检测小鼠脾脏中CD4+CD25+Foxp3+Treg的含量;培养小鼠骨髓来源的树突状细胞(DC),将DC与脾T淋巴细胞混合培养,应用ELISA法检测脾T淋巴细胞干扰素γ(IFNγ)的分泌量;同时测量肿瘤的大小,绘制肿瘤生长曲线,并观察各组荷瘤小鼠自身免疫病的发生情况。结果CTX的最佳应用剂量为100mg/kg。随着荷瘤时间的延长,荷瘤小鼠脾脏CD4+CD25+Foxp3+/CD4+的比值呈逐渐升高趋势。单次应用CTX抑制Treg的时间较短,而循环应用CTX能够延长对Treg的抑制,使其维持在相对较低的水平;循环应用CTX显著提高了荷瘤小鼠脾T淋巴细胞IFNγ的分泌水平。单次和循环应用CTX均未能延缓肿瘤的生长,两组小鼠均未见免疫性白斑及明显的化疗毒副反应。结论循环应用CTX能更加有效地调控Treg,从而促进DC对T淋巴细胞的抗原特异性激活,这将会提高DC疫苗的抗瘤效果。
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| 关 键 词： | 环磷酰胺 黑色素瘤 调节性T细胞 树突状细胞 |
Influence of Cyclical Administration with Low-dose Cyclophosphamide on Regulatory T Cells of Melanoma-bearing Mice and Its Antitumor Effect |
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| LI Wei-bo,YIN Yu,CAI Jian-hui,et al.Influence of Cyclical Administration with Low-dose Cyclophosphamide on Regulatory T Cells of Melanoma-bearing Mice and Its Antitumor Effect[J].Chinese Journal of Biologicals,2010,23(4). |
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| Authors: | LI Wei-bo YIN Yu CAI Jian-hui |
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| Affiliation: | LI Wei-bo,YIN Yu,CAI Jian-hui,et al (Second Hospital of Hebei Medical University,Shijiazhuang 050051,China) |
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| Abstract: | Objective To observe the influence of cyclical administration with low-dose cyclophosphamide(CTX)on regulatory T cells(Treg)of melanoma-bearing mice and its antitumor effect. Methods Melanoma-bearing mouse model was established by inoculating s.c. mice with melanoma B16 cells. The model mice were injected i.p. with CTX once or every 7 d for 3 times, and determined for CD4+CD25+Foxp3+ Treg content in spleens by flow cytometry. The onsets of autoimmune diseases of model mice in various groups were observed. M... |
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| Keywords: | Cyclophosphamide(CTX) Melanoma Regulatory T cells(Treg) Dendritic cells(DCs) |
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