RhoC基因沉默对肝癌细胞Bel7402凋亡和迁移的影响

目的研究RhoC基因沉默对肝癌细胞Bel7402凋亡和迁移的影响。方法以RNAi沉默Bel7402细胞RhoC基因的表达,FACS和RT-PCR检测细胞凋亡和凋亡相关基因水平,细胞划痕损伤和软琼脂克隆形成试验检测细胞迁移和生长特性。结果RNAi组细胞凋亡率明显高于细胞对照组,RNAi组Bcl-2表达水平明显低于细胞对照组,只有RNAi组可检测到Bax基因的表达。两对照组细胞划痕损伤在48h内愈合,而RNAi组则不能修复。RNAi组软琼脂克隆形成能力明显低于两对照组。结论RhoC基因沉默能够促进肝癌细胞Bel7402凋亡并抑制细胞迁移和非锚定生长能力。

Effect of RhoC Gene Silencing on Apoptosis and Migration of Hepatocellular Carcinoma Bel7402 Cells

Objective To study the effect of RhoC gene silencing on apoptosis and migration of hepatocellular carcinoma Bel7402 cells. Methods Silence the expression of RhoC gene in Bel7402 cells by RNAi. Determine the levels of apoptosis gene and apoptosis-related gene by FACS and RT-PCR, and the migration and growth of cells by scarification healing test and soft agar clone formation test. Results The apoptosis rate of cells transfected with recombinant plasmid containing RNAi was significantly higher than that with control plasmid, and the expression level of Bcl-2 gene in the former was significantly lower than that in the latter. The expression of Bax gene was observed in the cells transfected with recombinant plasmid containing RNAi. The scratch of cells transfected with control plasmid was healed within 48 h, but was not in those with recombinant plasmid containing RNAi. The formation rate of soft agar clones of cells transfected with recombinant plasmid containing RNAi was significantly lower than that with control plasmid. Conclusion RhoC gene silencing promotes the apoptosis and inhibits the migration and anchor independent growth of hepatocellular carcinoma Bel7402 cells.