Application of high-performance size-exclusion chromatography to study the autoxidation of unsaturated triacylglycerols |
| |
Authors: | Anu I Hopia Anna-Maija Lampi Vieno I Piironen Lea E T Hyvönen Pekka E Koivistoinen |
| |
Affiliation: | (1) Department of Food Technology, University of Helsinki, Helsinki, Finland;(2) University of Helsinki, Department of Applied Chemistry and Microbiology, Food Chemistry Division, University of Helsinki, P.O. Box 27, SF-00014, Finland |
| |
Abstract: | A combination of solid-phase extraction (SPE) and high-performance size-exclusion chromatography (HPSEC) was used to study
the autoxidation of triacylglycerol (TAG) mixtures separated from low-erucic acid rapeseed oil and butter oil. The samples
were autoxidized in the dark at 40°C for four weeks. The polar compounds of the autoxidated samples were separated by SPE
(NH2 stationary phase), and the polar fraction was further characterized by HPSEC with a series of three size-exclusion columns
and an evaporative light-scattering detector. The polar fraction contained TAG polymers, polar TAG monomers (PTAG) and diacylglycerols.
Peroxide values and anisidine values of the samples were also measured. By using three different types of TAG mixtures, it
could be demonstrated that the PTAG content of the TAGs increases during autoxidation. A slight increase was also detected
in polymer content. The correlation between PTAG content and the comparative measurements was considered significant. The
results indicate that the measurement of PTAG and polymeric material content by HPSEC analysis can be used when studying the
autoxidation level of edible oils and in characterizing the autoxidation products of different molecular sizes. |
| |
Keywords: | Autoxidation edible oils high-performance size-exclusion chromatography solid-phase extraction |
本文献已被 SpringerLink 等数据库收录! |
|