N3-Kethoxal-Based Bioorthogonal Intracellular RNA Labeling |
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| Authors: | Jinguo Huang Ruiqi Zhao Jing Mo Fang Wang Dr Xiaocheng Weng Xiang Zhou |
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| Affiliation: | 1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei, 430072 P. R. China;2. Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071 P. R. China |
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| Abstract: | There is growing interest in developing intracellular RNA tools. Herein, we describe a strategy for N3-kethoxal (N3K)-based bioorthogonal intracellular RNA functionalization. With N3K labeling followed by an in vivo click reaction with DBCO derivatives, RNA can be modified with fluorescent or phenol groups. This strategy provides a new way of labeling RNA inside cells. |
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| Keywords: | azides bioorthogonal intracellular probes RNA |
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