| 无启动子转化策略用于分离组织特异性启动子的研究 |
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| 引用本文： | 吴旭乾,何光源.无启动子转化策略用于分离组织特异性启动子的研究[J].湖北化工,2012(6):56-58,61. |
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| 作者姓名： | 吴旭乾 何光源 |
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| 作者单位： | [1]武汉软件工程职业学院环境与生化工程系,湖北武汉430205 [2]华中科技大学生命学院,湖北武汉430074 |
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| 基金项目： | 武汉市教育局教科研课题（2009165） |
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| 摘 要： | 通过研究外源uidA基因在体内表达的时空特异性,利用PCR、GUS组织化学检测等技术,从以tritordeum为受体、经无启动子uidA转化策略得到的转基因供试材料中成功筛选出了外源uidA基因在花药原基特异表达的单株。结果表明,花药原基特异表达单株的内源组织特异性启动子已被外源uidA片段标定,为启动子的分离克隆提供了一种有效手段。
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| 关 键 词： | tritordeum 启动子 花药原基 uidA |
Research of the Non-Promoter Transformation Tactic Used to Isolating the Tissue-Specific Promoter |
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| WU Xu-qian,HE Guang-yuan.Research of the Non-Promoter Transformation Tactic Used to Isolating the Tissue-Specific Promoter[J].Hubei Chemical Industry,2012(6):56-58,61. |
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| Authors: | WU Xu-qian HE Guang-yuan |
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| Affiliation: | 1. Department of Environment and Bio-Chemistry Engineering ,Wuhan Vocational College of Software and Engineering , Wuhan 430205,China; 2. College of Life Science, Huazhong University of Science and Technology ,Wuhan 430074, China) |
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| Abstract: | Through researching the space-time expression character of inserted foreign uidA, the single strain with anther-primordium tissue-specific expression of foreign uidA was screened successfully using PCR and GUS from the transgenic materials obtained by the non-promoter transformation tactic using tritordeum as acceptor. The method provided an effective measure for separating and cloning promoter. |
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| Keywords: | tritordeum promoter anther-primordium uidA |
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