HPLC法测定醒脑再造胶囊中盐酸小檗碱的含量

建立高效液相色谱法测定醒脑再造胶囊中盐酸小檗碱含量的方法。采用Hypersil-BDS色谱柱,以磷酸盐缓冲液(0.05 mol/L磷酸二氢钾和0.05 mol/L庚烷磺酸钠(1∶1),含0.2%三乙胺,并用磷酸调pH值至3.0)∶乙腈(60∶40)为流动相,检测波长为265 nm。盐酸小檗碱在0.04404~1.3212μg范围内与峰面积呈良好的线性关系,相关系数为1.0000,平均回收率为97.20%(RSD=1.43%)。该法快速简便,精确度好,可作为控制醒脑再造胶囊质量的方法。

Determination of Berberine Hydrochloride in Xingnao Zaizao Jiaonang Capsule by HPLC

The berberine hydrochloride in Xingnao Zaizao Jiaonang was determiued by HPLC method.The HPLC system consisting of an Hypersil-BDS column with detection wavelength at 265 nm and a mobile phase of phosphate buffer0.05 mol/L potassium dihydrogen phosphate,and 0.05 mol/L sodium heptane(1∶1),containing 0.2% triethylamine,and adjusting with phosphoric acid to pH 3.0]-acetonitrile(60∶40) was used.Good linearity of berberine hydrochloride was obtained(r=1.0000) within the range of(0.04404)～1.3212 μg.The average recovery was 97.20%;the repeatability was good;and RSD was 1.43%.This method was proved to be simple, rapid,accurate and sensitive,and it could be used for drug control.