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| 复合核苷氧钒配合物(VRC)的合成及对RNA酶的抑制效应 | |
| 引用本文： | 薛德平,颜世庆,王琰,林文玉,白桃,王申五,张礼和,王夔.复合核苷氧钒配合物(VRC)的合成及对RNA酶的抑制效应[J].化学试剂,2001,23(1):1-5,31. |
| 作者姓名： | 薛德平 颜世庆 王琰 林文玉 白桃 王申五 张礼和 王夔 |
| 作者单位： | 1. 南京大学化学化工学院, 2. 北京医科大学人民医院中心实验室, 3. 北京医科大学 |
| 摘 要： | 运用改进的有机溶剂助溶法合成了复合核苷氧钒配合物（VRC）——一种强的核糖核酸酶（RNAase)抑制剂，并经紫外差谱、红外光谱、顺磁共振光谱研究了核苷与氧钒离子在不同pH值和不同金属/核苷比中的结合及结合方式。实验结果表明，用本法制备的VRC在保护核糖核酸免受核糖核酸酶的降解方面与国外同类产品具有同样的效能，与蛋白类的核糖核酸酶抑制剂RNAasin相比，可在较高的温度下发挥核糖核酸酶抑制剂的作用。 |
| 关 键 词： | RNA酶 抑制效应 复合核苷氧钒配合物 合成 抑制剂 核糖核酸酶 RNA 水解 |
| 文章编号： | 0258-3283(2001)01-0001-05 |
Synthesis of vanadyl-ribonucieosid e complexes (VRC) and their inhibition on ribonuclease activity |
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| Xue Deping,Yan Shiqing,Wang Yan,Lin Wenyu,Bai Tao,Wang Shenwu,Zhang Lihe,Wang Kui.Synthesis of vanadyl-ribonucieosid e complexes (VRC) and their inhibition on ribonuclease activity[J].Chemical Reagents,2001,23(1):1-5,31. | |
| Authors: | Xue Deping Yan Shiqing Wang Yan Lin Wenyu Bai Tao Wang Shenwu Zhang Lihe Wang Kui |
| Abstract: | A modified method was proposed to synthesize vanadyl-ribonuleoside complexes (VRC),a strong ribonuclease inhibitor by the interaction of VO2+ with four ribonuclesides. The composition, binding mode and the inhibitive capacity of VRC were investigated at varied pH values and metal-to-ligand ratios by differential UV- absorption spectroscopy, IR spectroscopy and ESR. The activity of the VRC thus prepared was shown to be comparable with Bio-Labs' product in protecting RNA against RNase. The comparative experiments indicated that the synthesized VRC showed higher inhibitive activity than RNasin at a higher temperature. |
| Keywords: | vanadyl ribonucleoside ribonuclease (RNase) inhibition |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
