| 高效液相色谱测定鸡肝中洛克沙砷的方法研究 |
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| 引用本文： | 曹庆云,颜惜玲,周武艺,叶慧,管武太.高效液相色谱测定鸡肝中洛克沙砷的方法研究[J].应用化工,2005,34(10):640-643. |
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| 作者姓名： | 曹庆云 颜惜玲 周武艺 叶慧 管武太 |
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| 作者单位： | 华南农业大学动物科学学院 广东广州510642
(曹庆云，颜惜玲，叶慧)，华南农业大学理学院 广东广州510642
(周武艺)，华南农业大学动物科学学院 广东广州510642(管武太) |
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| 基金项目： | 留学人员回国基金资助项目(4300-C02003) |
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| 摘 要： | 建立了高效液相色谱(HPLC)测定鸡肝中洛克沙砷含量的实验方法。样品经匀浆、甲醇提取、高氯酸去蛋白后,用Nova-Pa k色谱柱(C18,150×3.9 mm,4μm)分离,以0.05 mol/L磷酸二氢钾∶甲醇∶10%乙酸(80∶10∶10)为流动相,在流速0.8 mL/m in,柱温30℃,进样量10μL的条件下,于264 nm波长处检测。结果表明,该方法的线性范围为0.50~10.0μg/mL,线性相关系数R2=0.9741,在两个添加浓度水平上的平均加标回收率分别为83.7%、89.9%。
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| 关 键 词： | 洛克沙砷 高效液相色谱 紫外检测 鸡肝 |
| 文章编号： | 1671-3206(2005)010-0640-04 |
| 修稿时间： | 2005年8月19日 |
Determination of roxarsone in chicken livers by high performance liquid chromatography |
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| CAO Qing-yun,YAN Xi-ling,ZHOU Wu-yi,YE Hui,GUAN Wu-tai.Determination of roxarsone in chicken livers by high performance liquid chromatography[J].Applied chemical industry,2005,34(10):640-643. |
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| Authors: | CAO Qing-yun YAN Xi-ling ZHOU Wu-yi YE Hui GUAN Wu-tai |
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| Affiliation: | CAO Qing-yun~1,YAN Xi-ling~1,ZHOU Wu-yi~2,YE Hui~1,GUAN Wu-tai~1 |
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| Abstract: | A high performance liquid chromatographic method(HPLC) was developed for the determination of roxarsone in chicken livers.The liver samples were subjected to homogenate and extraction by(using) methanol as extracting agent,and then HClO_4 was utilized to get rid of protein.The chromatographic separation was performed on a Nova-Pak~()C_(18)column(150×3.9 mm,4 μm) using 0.05 mol/L KH_2PO_4∶CH_3OH∶10%CH_3COOH(80∶10∶10) as mobile phase at a rate of 0.8 mL/min,temperature of 30℃,injection sample size of 10 μL and UV detection at 264 nm.The results demonstrated that the linear range was 0.50～10.0 μg/mL with linear relative coefficient 0.9741.The average recoveries of two different addition levels were 83.7% and 89.9% respectively. |
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| Keywords: | roxarsone HPLC UV detection chicken liver |
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