RP-HPLC-ELSD同时测定附子地上部分中新乌头碱和次乌头碱的含量

采用反相高效液相色谱-蒸发光散射监测器(RP-HPLC-ELSD)测定附子地上部分中新乌头碱和次乌头碱的含量。色谱柱为Krom asil C18(150 mm×4.6 mm,5μm),流动相为乙腈-0.1%乙二胺水溶液(体积比为60∶40),柱温为室温,流速1 mL/m in,漂移管温度89.0℃,气体流量2.60 L/m in。结果表明,新乌头碱和次乌头碱均得到较好分离,均在12.5~400μg/mL呈现良好的线性关系;平均加样回收率分别为100.01%(RSD=0.31%),99.53%(RSD=0.51%)。RP-HPLC-ELSD法具有准确度高,简便快捷,干扰少,重现性好的优点,可用于附子地上部分中双酯型生物碱的测定。

Determination of mesaconitine and hypaconitine in the aerial part of radix aconiti lateralis praeparata by RP-HPLC-ELSD

The content of mesaconitine and hypaconitine in the aerial part of radix aconiti lateralis praeparata by RP-HPLC-ELSD.Two constituents were analyzed simultaneously with a Kromasil C18 column(150 mm×4.6 mm,5 μm) by isocratic elution using acetonitrile-0.1% ethylenediamine(60 ∶40) as the mobile phase.The column was maintained at room temperature.The flow rate was 1 mL/min.The detector drift tube was kept at 89 ℃,the nebulizing gas flow-rate was 2.6 L/min.Excellent chromatographic separation was achieved for mesaconitine and hypaconitine.The liear ranges of the two constituents were both 12.5~400 μg/mL.The extraction recoveries were 100.01%(RSD=0.31%),99.53%(RSD=0.51%),respectively.The method has the advantages of accuracy,simplicity,and precision,it is suitable for quantitative analysis of mesaconitine and hypaconitine in radix aconiti lateralis praeparata.