HPLC法同时测定化妆品中的12种防腐剂

建立了化妆品中12种常用防腐剂(甲基氯异噻唑啉酮、2-溴-2-硝基丙烷-1,3-二醇、甲基异噻唑啉酮、苯甲醇、苯氧乙醇、苯甲酸、4-羟基苯甲酸甲酯、4-羟基苯甲酸乙酯、4-羟基苯甲酸异丙酯、4-羟基苯甲酸丙酯、4-羟基苯甲酸异丁酯、4-羟基苯甲酸丁酯)的HPLC检测方法。采用Waters symmetry(250 mm×4.6 mm×5μm)色谱柱,流动相为乙腈-0.1%(体积分数)磷酸,梯度洗脱,柱温35℃,DAD检测器,以各物质的紫外光谱数据和保留时间定性。检测波长设定为:甲基氯异噻唑啉酮和甲基异噻唑啉酮在280 nm检测,其余成分在254 nm检测。结果发现,在所建立的方法下,12种防腐剂的分离度良好、线性范围广、准确度高。

Simultaneous Determination of Twelve Kinds of Preservative in Cosmetics by HPLC

To establish the simultaneous determination method of 12 kinds of common preservative （methyl chloride isothiazolinone,2-bromo-2-nitro-1,3-propanediol,methyl isothiazolinone,benzene methanol,phenoxyethanol,benzoic acid,methyl 4-hydroxy benzoate,ethyl 4-hydroxy benzoate,4-hydroxy benzoic acid isopropyl ester,propyl 4-hydroxy benzoate,4-hydroxy benzoic acid iso butyl ester,4-hydroxy benzoic acid butyl ester） in cosmetics by HPLC,Waters symmetry （250 mm× 4.6 mm × 5 μm） chromatographic column was used with mobile phase system of acetonitrile-0.1 ％ phosphate and gradient elution,column temperature was 35 ℃,and DAD detector was equipped.The samples were detected qualitatively with ultraviolet spectrum and retention time,the detection wavelength were：methyl chloride isothiazolinone and methyl isothiazolinne at 280 nm,the rest at 254 nm.The separation results of all samples were well with wide linear range and high accuracy.