In-vitro behavior of different fully dense calcium phosphate materials fabricated by Spark Plasma Sintering |
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| Affiliation: | 1. Dipartimento di Ingegneria Meccanica, Chimica e dei Materiali, Unità di Ricerca del Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM) – Università degli Studi di Cagliari, via Marengo 2, 09123 Cagliari, Italy;2. Dipartimento di Scienze Biomedic he, Sezione di Citomorfologia, Università degli Studi di Cagliari, Cittadella Universitaria di Monserrato, S.P. 8 Km 0.700, 09042 Monserrato, CA, Italy;1. School of Materials Science and Engineering, Anhui University of Technology, Maanshan 243032, PR China;2. Jiangsu Collaborative Innovation Center for Advanced Inorganic Function Composites, Nanjing Tech University, Nanjing 210009, PR China;3. Key Laboratory of Metallurgical Emission Reduction & Resources Recycling, Ministry of Education, Anhui University of Technology, Maanshan 243002, PR China;1. Instituto de Ciencia de Materiales de Aragón (ICMA), CSIC–Universidad de Zaragoza, E50018 Zaragoza, Spain;2. Centro Universitario de la Defensa, General Military Academy, Ctra. Huesca s/n, E50090 Zaragoza, Spain;1. Key Laboratory of Inorganic Functional Materials and Devices, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, People''s Republic of China;2. University of Chinese Academy of Sciences, Beijing 100049, People''s Republic of China;1. Lecturer, Specialist in Oral and Maxillofacial Surgery, Research Group of Tissue Engineering, Medical Research Center, University of Oulu, Oulu, Finland;2. Biostatistician, Department of Radiology, Oulu University Hospital, Oulu, Finland;3. Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, Oulu Center for Cell-Matrix Research, University of Oulu, Oulu, Finland;4. Department of Anatomy and Cell Biology, Cancer Research and Translational Medicine Research Unit, University of Oulu Respiratory Research Unit, Department of Internal Medicine and Medical Research Center, Oulu University Hospital, Oulu, Finland;5. Director of Cleft Lip and Palate Program, Oulu University Hospital and Medical Research Center, University of Oulu, Oulu, Finland;6. Professor of Pediatric Surgery, Department of Children and Adolescents, Division of Pediatric Surgery, Oulu University Hospital, Medical Research Center, PEDEGO Research Center, University of Oulu, Oulu, Finland;7. Professor of Tissue Engineering and Oral and Maxillofacial Surgery, Medical Research Center, University of Oulu and Oulu University Hospital, Oulu, Finland; Director of Research, BioMediTech, Institute of Biosciences and Medical Technology, University of Tampere, Tampere, Finland;1. Inst. Ciencia de Materiales de Sevilla (CSIC-US), Américo Vespucio 49, 41092 Sevilla, Spain;2. Dpto. de Física de la Materia Condensada. Univ. de Sevilla, Apdo. 1065, 41080 Sevilla, Spain |
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| Abstract: | Results obtained during in-vitro experiments concerning human osteoblasts cultivated on the surface of dense samples produced by Spark Plasma Sintering from three types of hydroxyapatite powders are described and discussed. The sintered products display diverse composition and microstructures which are found to significantly influence the biological response of the cells. Osteoblasts adhesion, viability and proliferation are quantitatively comparable for the three classes of bioceramics, whereas matrix mineralization occurs only in products exclusively consisting of hydroxyapatite. Correspondingly, a calcium-phosphate layer exhibiting a trabecular-like microstructure is deposited on the materials surface. Matrix mineralization is favored when the substrate is composed of submicrometer-sized apatite grains. On the other hand, the latter phenomena is markedly suppressed, and so does the formation of the new apatite phase, when cells are seeded on sintered disks composed of β-Tri-Calcium Phosphate, which was formed during the sintering process from the decomposition of initial apatite. |
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| Keywords: | Osteoblasts Mineralization Calcium phosphates Spark Plasma Sintering Microstructure |
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