| 高效液相色谱法测定湖水中的微囊藻毒素 |
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| 引用本文： | 蒋瑶,杨卫花,赵浩军,杨朝义,李颖,寸宇智.高效液相色谱法测定湖水中的微囊藻毒素[J].云南化工,2018(2):66-68. |
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| 作者姓名： | 蒋瑶 杨卫花 赵浩军 杨朝义 李颖 寸宇智 |
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| 作者单位： | 大理大学农学与生物科学学院;大理州质量技术监督综合检测中心 |
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| 摘 要： | 建立一种测定湖水中微囊藻毒素的高效液相色谱法(high performance liquid chromatography,HPLC)。以0.5mL/L三氟乙酸水溶液作为流动相,Agilent Eclipse XDB C18柱分离,紫外检测器(VWD)在波长238nm处检测。本方法最低检出限是0.4μg/L。微囊藻毒素标准浓度在0.4μg/mL-2μg/mL范围内,线性关系良好,相关系数r达0.998。加标浓度为0.4μg/mL-1.8μg/mL时,回收率为84.2%-92.0%,相对标准偏差在0.2%-3.2%之间。该方法灵敏度、准确度高,操作简单、安全。可用于测定水体中的微囊藻毒素。
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| 关 键 词： | 高效液相色谱法 HLR固相萃取柱 水质检测 水华 微囊藻毒素 |
Determination of Microcystin in Lake Water by HPLC |
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| Affiliation: | ,College of Agriculture and Bioscience,Dali University,Dali Quality and Technical Supervision Comprehensive Inspection Center |
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| Abstract: | In this study,we intended to develop a method for the determination of microcystin in lake water by high performance liquid chromatographic(HPLC). The trichloroacetic acid solution with 0.5 m L/L was applied as mobile phase. Microcystin was separated by Agilent Eclipas XDB C18 column and determined at 238 nm wavelength by VWD. The detection limit of the method reached 0.4μg/L. There was a good linearity within the range of 0.4μg/m L to 2μg/m L with the correlation coefficient of 0.998. The recoveries were between 84.2% and 92.0% at the spiking level of 0.4μg/m L to 1.8μg/m L,and the relative standard deviation ranged from 0.2% to 3.2%. The method built in this study is sensitive,accurate and easy to operate. |
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| Keywords: | HLR solid-phase extraction column HPLC water quality testing microcystin water blooms |
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