Development of modified stokes expression to model the behavior of expanded beds containing polydisperse resins for protein adsorption |
| |
Authors: | Yong?Rae?Chae Yeo?Joon?Yoon Email author" target="_blank">Keun?Garp?RyuEmail author |
| |
Affiliation: | (1) School of Chemical Engineering and Bioengineering, University of Ulsan, 680-749 Ulsan, Korea;(2) Division of Nano Sciences and Department of Chemistry, Ewha Womans University, 120-750 Seoul, Korea |
| |
Abstract: | Expanded bed behavior was modeled by using the Richardson-Zaki correlation between the superficial velocity of the feed stream and the void fraction of the bed. A polydisperse material, Chelating excellose® (70-210 Μm in diameter, 1.21 g/cm3 in density), which has Ni2+ ions for the selective binding of histidine-tagged proteins, was used as the resin. A method to modify the Stokes expression to express the terminal settling velocity of the resins by introducing two empirical parameters, the effective diameter of the resins and an exponent for(ρ p -ρ)/Μ term, was developed. Combined use of the Richardson-Zaki correlation and the modified Stokes expression was successful in modeling the bed expansion by incorporating physical properties of feed streams and the resins. |
| |
Keywords: | Expanded Bed Modeling Scale-up Stokes Expression |
本文献已被 SpringerLink 等数据库收录! |
|