高效液相色谱法同时测定工业废水中的2,4-二叔丁基酚和2,6-二叔丁基酚

采用高效液相色谱法同时测定工业废水中的2,4-二叔丁基酚和2,6-二叔丁基酚的含量。色谱条件为ZORBAX -XDB-C8色谱柱（4.6 mm×150 mm,5μm）,流动相为水：甲醇：异丙醇（25＋60＋15）混合液，流量为1.0 mL·min-1，进样量20μL,调节检测波长为270 nm。在该方法下2,4-二叔丁基酚和2,6-二叔丁基酚的线性范围为0.005~0.5 mg·mL-1（相关系数分别为0.9999, n=5；0.9999, n=5）。加标回收率分别在98.3%~101.8%和98.5%~101.0%之间，相对标准偏差（n=8）分别为1.35%和1.07%。所用的方法准确简便、重复性好，可作为2,4-二叔丁基酚和2,6-二叔丁基酚含量测定的有效方法。

Determination of 2, 4-Di-tert-butylphenol and 2, 6-Di-tert-butylphenol in Industrial Wastewater by HPLC

Determination of 2,4-Di-tert-butylphenol and 2,6-Di-tert-butylphenol in ind-ustrial wastewater by HPLC was developed. Chromatographic conditions included an ZOR-BAX-XDB-C8 column （4.6mm ×150mm, 5μm） with the mobile phase consists of water： methanol：isopropanol （25＋60＋15）, The UV detection wavelength was set up at 270 nm and 20 μL samples were injected. In this method, 2,4-Di-tert-butylphenol and 2,6-Di-tert-butylphenol linear range were 0.005~0.5 mg·mL-1 （r=0.9999,n=5;0.9999,n=5） and the standard addition recovery were from 98.3% to 101.8% and from 98.5% to 101.0%, the RSD were 1.35% and 1.07%（n=8）. The method is simple, accurate, reliable and suitable for quality controlof 2,4-Di-tert-butylphenol and 2,6-Di-tert-butylphenol.