Towards the detection of human papillomavirus infection by a reagentless electrochemical peptide biosensor |
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| Authors: | B Piro A Kapella VH Le G Anquetin QD Zhang S Reisberg V Noel LD Tran HT Duc MC Pham |
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| Affiliation: | aLaboratoire Interfaces-Traitements-Organisation et Dynamique des Systèmes (ITODYS), Associé au CNRS-UMR 7086, Université Paris Diderot – Paris 7, 15 rue Jean-Antoine de Baïf, 75205 Paris Cedex 13, France;bUniversité Paris XI, INSERM U-1014, Groupe Hospitalier Paul Brousse-94800 Villejuif, France;cInstitute of Materials Sciences, VAST, 18 av. Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam |
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| Abstract: | In the present work, we report first results about a technology using a conjugated copolymer poly(5-hydroxy-1,4-naphthoquinone-co-5-hydroxy-2-carboxyethyl-1,4-naphthoquinone) acting both as immobilizing and transducing element for reagentless immunosensor, and its application for the detection of HPV infection. It was shown that the reagentless immunosensor was able to detect the interaction between antigenic peptide L1 from HPV-16 major capsid protein, a dominant epitope involved in viral infection as well as in prophylactic vaccine, and the relevant antibody. |
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| Keywords: | Conjugated polymer Electrochemical immunosensor Reagentless detection Peptide&ndash antigen affinity ELISA assays Human papillomavirus |
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