| 离子交换-高效液相色谱法测定化妆品中6种水溶性功效成分 |
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| 引用本文： | 夏泽敏,招启文,沈聪文,李慧怡,席绍峰.离子交换-高效液相色谱法测定化妆品中6种水溶性功效成分[J].广东化工,2014(13):277-278. |
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| 作者姓名： | 夏泽敏 招启文 沈聪文 李慧怡 席绍峰 |
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| 作者单位： | 广州市质量监督检测研究院国家化妆品质量监督检验中心(广州),广东广州510110 |
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| 摘 要： | 建立了一种同时测定化妆品中6种水溶性功效成分的分析方法。样品经水直接超声提取后经离子交换色谱柱Rezex ROA-Organic Acid H+(8%)(300×7.8 mm)分离,10 mmol/L硫酸水溶液作为流动相,流速为0.5 mL/min,二极管阵列检测器进行检测,检测波长分别254 nm和214 nm,外标法定量。在优化实验条件下,6种水溶性功效成分标准曲线线性关系良好,在空白化妆品中的加标回收率为87.3%~99.4%,相对标准偏差为2.8%~5.7%。
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| 关 键 词： | 化妆品 维生素A类化合物 高效液相色谱法 |
Determination of 6 Water-Soluble Compounds in Cosmetics by HPLC-DAD |
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| Xia Zemin,Zhao Qiwen,Chen Congwen,Li Huiyi,Xi Shaofeng.Determination of 6 Water-Soluble Compounds in Cosmetics by HPLC-DAD[J].Guangdong Chemical Industry,2014(13):277-278. |
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| Authors: | Xia Zemin Zhao Qiwen Chen Congwen Li Huiyi Xi Shaofeng |
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| Affiliation: | (Guangzhou Products Quality Supervision and Testing Institute, National Center for Quality Supervision and Testing of Cosmetics (Guangzhou), Guangzhou 510110, China) |
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| Abstract: | A method was developed for the determination of 6 water-soluble compounds in cosmetics. The sample was extracted by methanol and separated by a RP Cs chromatographic column （4.6×250 mm, 5μm） at a flow rate of 1.0 mL/min. Methanol-water was used as mobile phase. The detection was performed at 325 nm by using high performance liquid chromatography-diode array detection （HPLC-DAD）. The external standard method was employed for quantitative analysis. Under the optimized condition, the average recoveries of spiked samples were between 87.3 % and 99.4 %, with relative standard deviations of 2.8 %-5.7 %. |
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| Keywords: | cosmetics Vitamin A compounds high performance liquid chromatography （HPLC） |
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