GC-MS法测定茶叶中的有机磷残留

建立了固相萃取净化-气相色谱质谱法测定茶叶中有机磷农药残留的分析方法。样品用乙腈作为提取溶剂,经Carb/NH2柱净化后供GC-MS分析。方法检出限为0.0050~0.020 mg/kg,在加标水平为0.10 mg/kg时,回收率为85.7%~101.5%,相对标准偏差为3.6%~8.6%。方法具有萃取效率高、净化效果好、精密度好,分析快速等优点,适用于茶叶中有机磷农药残留的分析检测。

Determination of Organophosphrous Pesticides Residue in Tea Using SPE Purification GC-MS Analysis

A method for determination organophosphrous pesticides residues in tea sample was described by solid phase extraction purification gas chromatography-mass spectrometr. The target analytes were extracted with the solution of acetonitrilc using accelerated solvent extraction, purified using Carb/NH2 solid phase extraction. The separation and determination of analytes were performed by using GC-MS with selected ion monitoring（SIM） mode and external standard method. Under the optimal conditions, the detection limits（S/N=3） of pesticides were in the range of 0.0050-0＋020 mg/kg with the relative standard deviations of 3.6 %-8.6 %. The recoveries of pesticides from teas sample spiked with mixed standards at 0.10 mg/kg ranged from 85.7 %-101.5 %. The method was rapid, precise, sensitive, high efficient in extraction convenient and purification. After routine applications, the results indicated that this method is suitable for the determination of organophosphrous pesticides resdues in the tea.