汉江上游干支流沉积物细菌群落多样性和影响因素

为了解汉江上游干支流沉积物细菌多样性以及确定性过程和随机性过程在沉积物细菌群落构建过程中的相对重要性,基于Illumina高通量测序技术,分析了环境因子对细菌群落组成的影响,采用非度量多维尺度(NMDS)排序探究了季节之间沉积物细菌群落的差异,并结合中性群落模型和标准化随机率量化了确定性过程和随机过程对群落构建的影响。结果表明:汉江上游及其支流细菌群落主要由变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、蓝藻门(Cyanophyta)、浮霉菌门(Planctomycetes)和酸杆菌门(Acidobacteria)等组成；细菌群落在不同季节有显著差异；地理距离和环境因子对细菌群落结构影响较小,确定性过程并未在细菌群落组成中起到主导作用；随机过程很大程度上影响了群落在秋季和春季的组成,是沉积物细菌群落构建的主导因素。

Diversity and influencing factors of bacterial community in sediments of upper reaches of the Hanjiang River and its tributaries

In order to understand the sediment bacterial diversity of the upper reaches of the Hanjiang River and its tributaries, as well as the relative importance of deterministic and stochastic processes in the construction of sediment bacterial community, the impact of environmental factors on bacterial community composition was analyzed based on the Illumina high-throughput sequencing technology, the difference of sediment bacterial communities in different seasons was investigated using the non-metric multidimensional scaling (NMDS) method, and the impact of deterministic and stochastic processes on community construction was quantified by combining the neutral community model and the normalized stochasticity ratio. The results show that the bacterial communities in the upper reaches of the Hanjiang River and its tributaries are mainly composed of Proteobacteria, Bacteroidetes, Cyanophyta, Planctomycetes, and Acidobacteria. Bacterial communities differ significantly in different seasons. Geographical distance and environmental factors have little effect on the structure of bacterial communities, and the deterministic process did not play a leading role in bacterial community composition. The stochastic process greatly affected the composition of the community in autumn and spring, and the stochastic process is the dominant factor in the construction of sediment bacterial community.