| 痰热清注射液对慢性髓系白血病K562细胞增殖的抑制作用 |
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| 引用本文： | 党艳辉,李薇,杨波,卢学春,黄玉,张峰,朱宏丽.痰热清注射液对慢性髓系白血病K562细胞增殖的抑制作用[J].压电与声光,2010(5). |
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| 作者姓名： | 党艳辉 李薇 杨波 卢学春 黄玉 张峰 朱宏丽 |
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| 作者单位： | 解放军总医院南楼血液科;吉林大学第一医院血液肿瘤中心;中国科学院北京基因组研究所; |
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| 基金项目： | 军队“十一·五”基金项目(2007增补)~~ |
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| 摘 要： | 目的观察痰热清注射液对慢性髓系白血病K562细胞增殖的抑制作用。方法将痰热清注射液倍比稀释成1:2、1:4、1:8、1:16、1:32、1:64、1:128、1:256、1:512共9个浓度组,分别处理增殖期慢性髓系白血病K562细胞,镜下观察不同时间点各浓度组细胞生长情况,应用CCK-8法检测细胞增殖能力,绘制生长曲线,计算抑制率和半数抑制浓度(IC50)。结果痰热清注射液能明显抑制K562细胞增殖,细胞倍增时间为24h,1:2至1:8稀释浓度组的细胞毒性大,细胞基本死亡;抑制作用呈剂量和时间依赖性,IC50约为1:55稀释浓度。结论痰热清注射液对K562细胞具有抑制作用,这为临床应用痰热清注射液治疗血液肿瘤并发感染提供了实验基础。
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| 关 键 词： | 痰热清注射液 白血病 髓样 慢性期 K562细胞 细胞增殖 |
Inhibitory effect of Tanreqing injection on proliferation of chronic myelogenous leukemia K562 cell line |
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| DANG Yan-hui,LI Wei,YANG Bo,LU Xue-chun,HUANG Yu,ZHANG Feng,ZHU Hong-li.Inhibitory effect of Tanreqing injection on proliferation of chronic myelogenous leukemia K562 cell line[J].Piezoelectrics & Acoustooptics,2010(5). |
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| Authors: | DANG Yan-hui LI Wei YANG Bo LU Xue-chun HUANG Yu ZHANG Feng ZHU Hong-li |
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| Affiliation: | DANG Yan-hui1,2,3,LI Wei2,YANG Bo1,LU Xue-chun1,HUANG Yu1,ZHANG Feng3,ZHU Hong-li1 1Department of Geriatric Hematology,Chinese PLA General Hospital,Beijing 100853,China,2Department of Hematology , Oncology,Affiliated First Hospital of Jilin University,Changchun 130021,3Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing 100029 |
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| Abstract: | Objective To investigate the inhibitory effects of Tanreqing(phlegm and heat clearing) injection on proliferation of chronic myelogenous leukemia K562 cell line.Methods Tanreqing injection was diluted to 9 groups at the concentration of 1:2,1:4,1:8,1:16,1:32,1:64,1:128,1:256 and 1:512,respectively.Proliferating chronic myelogenous leukemia K562 cells were treated with Tanreqing injection at different dilution concentrations and their growth was observed at different time points under a microscope.CCK-8 assa... |
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| Keywords: | Tanreqing Injection Leukemia Myelogenous Chronic-Phase K562 Cell Cell Proliferation |
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