葛根素对血管性痴呆大鼠海马中低氧诱导因子-1α和红细胞生成素表达的影响

目的观察葛根素对血管性痴呆(VD)大鼠海马中低氧诱导因子-1α和红细胞生成素表达的影响并探讨其可能机制。方法采用大鼠双侧颈总动脉永久性阻断法(2-VO)建立VD的动物模型,SD大鼠随机分为假手术组、2-VO组和葛根素组,每组又分为1周、3周、6周、2月(n=6)四个时间点;应用Y型水迷宫实验测定动物的学习记忆能力,采用免疫组化法检测大鼠海马CA1区HIF-1α和EPO蛋白的表达。结果①2-VO组和葛根素组大鼠游全程时间和误入盲端次数均显著增加,但各时间点葛根素组大鼠的学习记忆成绩均显著优于2-VO组。②各时间点,2-VO组和葛根素组大鼠海马CA1区HIF-1α和EPO的表达均较假手术组明显增高,但葛根素组两种蛋白的表达均较2-VO组明显降低。结论葛根素可提高缺血脑组织细胞内氧浓度,改善学习记忆功能,对VD具有确切的保护作用。

Effects of puerarin on the expression of hypoxia inducible factor- 1α and erythropoietin in the hippocampus of vascular dementia rats

Objective To observe the effects of puerarin on the expression of hypoxia inducible factor-(1α)and erythropoietin in the hippocampus of vascular dementia rats and to investigate its possible mechanisms.Methods Rats of experimental group were treated with a permanent bilateral common carotid arteries(CCA) occlusion(2-VO) to establish vascular dementia model.Male SD rats were randomly divided into three groups: sham-operated group,2-VO group and puerarin group(twenty-four rats in each group).Rats were evaluated on learning-memory ability by Y-type water maze test.At corresponding time points at 1w,3w,6w and 2mon,the dynamic expression of HIF-1α and EPO in CA1 region of hippocampus was determined by immunohistochemical method.Results ① The swimming time and error times of rats in 2VO group and puerarin group were significantly increased;but at all time points the rats in puerarin group showed better performance than those in 2-VO group. ② Both in 2-VO group and puerarin group,the number of HIF-1α and EPO positive cells in hippocampal CA1 region were much more than that in sham-operated group;but the expression of HIF-1α and EPO at each point in puerarin group was distinctly less than that in 2-VO group.Conclusion Puerarin can increase the intracellular oxygen concentration and improve learning-memory ability during cerebral ischemia,and it has definitely protective effects on VD.