| Maxwell 16裂解纯化法提取陈旧精子DNA的应用 |
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| 引用本文： | 郭晓涛,郭建伟,杜勇,李勇,陈雪梅.Maxwell 16裂解纯化法提取陈旧精子DNA的应用[J].刑事技术,2014(5):12-13. |
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| 作者姓名： | 郭晓涛 郭建伟 杜勇 李勇 陈雪梅 |
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| 作者单位： | 1. 河南省鹤壁市公安局刑侦支队,458030
2. 广东省惠州市公安局刑侦支队,516000 |
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| 摘 要： | 目的利用Maxwell 16裂解纯化法从保存8年以上陈旧精斑检材中获取精子DNA。方法 8份陈旧精斑检材采用Maxwell 16裂解纯化法提取精子DNA,并采用Powerplex○R21试剂盒进行复合扩增,产物用AB3130型遗传分析仪检测,结果与常规方法进行对比。结果成功获得8份陈旧精斑检材精子STR分型。结论差异裂解配合Maxwell 16裂解在陈旧精斑检材精子DNA检验中效果明显。
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| 关 键 词： | 差异裂解 Chelex Maxwell 16 DNA提取 |
Application of Maxwell 16 in DNA extraction from old semen samples |
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| Affiliation: | OUO Xiao-tao, GUO Jian wei, DU Yong, et al (Public Security Bureau of Hebi City, Henan 458030, China) |
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| Abstract: | In this paper, DNA from 10 years old semen samples was extracted by using Maxwell 16 and the results were compared with conventional extraction methods. The study indicated that Maxwell 16 is effective for extracting DNA from old se men samples, |
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| Keywords: | Maxwell16 old semen extraction： DNA |
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