CellSense生物传感器毒性分析系统应用研究

介绍了一种基于大肠杆菌(Escherichia coli)的CellSense生物传感器毒性分析系统,对其毒性分析条件进行了初步优化,并探讨了其在重金属离子生物急性毒性分析中的应用性能.结果表明,以p-苯醌为电子传递介质时,基于大肠杆菌的CellSense生物传感器具有良好的毒性分析特性,测试得Hg2+、Cu2+、Zn2+和Ni2+四种金属离子的EC50分别为1.1、6.5、66.0和119.0μg·mL-1;呼吸基质的pH低于6.0和盐度(NaCl)高于3%(w/w)时,对E.coli的活性产生一定的抑制作用.

The Application Performance of CellSense Biosensor for Toxicity Assessment

A cost-effective strategy using an amperometric biosensor with Escherichia coli(E.coli)that provides a rapid toxicity determination of toxicants is described.The Cellsense biosensor system comprises a biological component immobilized in intimate contact with a transducer which converts the biochemical signal into a quantifiable electrical signal.It is showed that CellSense biosensor with E.coli has a good performance when p-benzoquinone is chosen as redox mediator.The 50% effective concentrations(EC50)values of Hg2+,Cu2+,Zn + and Ni2+ are 1.1,6.5,66.0 and 119.0μg·mL-1,respectively,which are in accordance with the results of conventional bacterial counting method.When the respiratory substrates pH is lower than about 6.0 and salinity(NaCl)is higher than 3%(w/w),the activity of E.coli is inhibited significantly.