| 基于DO-stat流加培养控制的L-异亮氨酸发酵条件优化 |
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| 引用本文： | 张俊丽,刘龙,房峻,李江华,堵国成,陈坚,宁健飞,蔡立明.基于DO-stat流加培养控制的L-异亮氨酸发酵条件优化[J].应用与环境生物学报,2011(3):317-320. |
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| 作者姓名： | 张俊丽 刘龙 房峻 李江华 堵国成 陈坚 宁健飞 蔡立明 |
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| 作者单位： | 江南大学生物工程学院;江南大学工业生物技术教育部重点实验室;江南大学食品科学与技术国家重点实验室;无锡晶海氨基酸有限公司; |
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| 基金项目： | 国家“973”计划项目(No.2007CB714306);“973”前期研究专项(No.2010CB535014); 国家杰出青年基金项目(No.20625619); 国家自然科学基金重点项目(No.20836003); 国家“863”计划项目(No.2007AA02Z229); 中央高校基本科研业务费专项(No.JUSRP30901)~~ |
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| 摘 要： | L-异亮氨酸(L-lie)产生菌代谢流的前期研究报道认为,较低的溶氧浓度有利于L-异亮氨酸的积累.为进一步提高L-异亮氨酸的产量,采用DO-stat流加培养控制方法,分别研究了溶氧浓度20%、30%、35%和40%下乳糖发酵短杆菌发酵液中葡萄糖浓度的变化以及L-异亮氨酸的合成情况.结果表明,当发酵罐中溶氧浓度控制在20...
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| 关 键 词： | L-异亮氨酸 溶氧(DO) DO-stat 补料分批发酵 乳糖发酵短杆菌 |
Optimization of L-isoleucine Fermentation Conditions Based on DO-stat Feeding Culture Strategy by Brevibacterium lactofermentation |
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| ZHANG Junli,LIU Long,FANG Jun,LI Jianghua,DU Guocheng,CHEN Jian,NING Jianfei & CAI Liming.Optimization of L-isoleucine Fermentation Conditions Based on DO-stat Feeding Culture Strategy by Brevibacterium lactofermentation[J].Chinese Journal of Applied and Environmental Biology,2011(3):317-320. |
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| Authors: | ZHANG Junli LIU Long FANG Jun LI Jianghua DU Guocheng CHEN Jian NING Jianfei & CAI Liming |
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| Affiliation: | ZHANG Junli1,2,LIU Long1,FANG Jun1,LI Jianghua1,DU Guocheng1,CHEN Jian3,NING Jianfei4 & CAI Liming4(1School of Biotechnology,Jiangnan University,Wuxi 214122,Jiangsu,China)(2Key Laboratory of Industrial Biotechnology,Ministry of Education,China)(3State Key Laboratory of Food Science and Technology,China)(4Wuxi Jinghai Amino Acid Co.Ltd.,Wuxi 214199,China) |
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| Abstract: | The dissolved oxygen(DO) concentration plays a significant role in the accumulation of amino acids during amino acid fermentation process.According to the known study on the metabolic flux of L-isoleucine producing strain,lower DO concentration was considered more desirable for L-isoleucine production.In this study,DO-stat feeding strategy was adopted to increase L-isoleucine production.Changes in glucose concentration and L-isoleucine formation under different DO concentrations,i.e.,20%,30%,35% and 40% wer... |
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| Keywords: | L-isoleucine dissolved oxygen(DO) DO-stat fed-batch fermentation Brevibacterium lactofermentation |
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