环境中的PAES对蟾蜍蝌蚪红细胞微核的影响研究

通过对邻苯二甲酸酯对蟾蜍蝌蚪红细胞微核的影响进行了研究。目的探讨邻苯二甲酸酯对蟾蜍蝌蚪的致突变作用。方法以邻苯二甲酸二甲酯为污染物对蟾蜍蝌蚪染毒24小时,并观察其细胞核变化。所用蟾蜍蝌蚪处于变态期,对污染物较为敏感。结果邻苯二甲酸酯使蟾蜍蝌蚪红细胞微核率以及核异常细胞率上升,微核率随着浓度的增大而上升,在浓度为78 ug/ml,对照组相比有极显著差异P〈0.01,浓度在18.5 ug/ml时试验组突变的细胞核的形态发生显著改变,核异常细胞率与对照组核异常细胞率有显著差异P〈0.05。此时显微镜下观察可见微核、双核、核内凹、核碎裂、无丝分裂时核分裂不均等核的形态变化。结论邻苯二甲酸酯对蟾蜍蝌蚪具有明显的致突变作用。

Studies on the Influence of PAES in the Environment on the Micronucleus Toad Tadpoles

We study the effects of the phthalate esters on China toad tadpoles micronucleus.Objective to identify the mutagenicity of phthalates toad tadpoles;Methods expose dimethyl phthalate micro-pollutants on toad tadpoles 24 hours,and observe the changes in their nuclei.Toad tadpoles we used are in the period of metamorphosis,which are to be more sensitive to pollutants;Results phthalates make toad tadpoles micronucleus rate and abnormal cell nuclear rise,micro-rate increases with the concentration rising from the concentration of 78 ug/ml to the control group（P〈0.01） and reaches a maximum level.Concentration in 18.5 ug/ml when the abnormal cell nuclear rise the control group is of significant differences（P〈0.05） levels.Mutations in the nuclear morphogenetic change significantly,through the microscopic observation we find the micronucleus,double-nuclear,nuclear eight characters,concave nuclear and nuclear fragmentation,no mitosis when nuclear fission uneven.Conclusion phthalates toad tadpole can affect micronucleus obviously.