Cu~(2+)和Zn~(2+)对日本新糠虾生长、蛋白含量和体内磷酸酶活性的影响

观察了不同浓度(10、20、40和80 μg/L)重金属离子Cu~(2+)和Zn~(2+)对日本新糠虾存活、蜕皮、蛋白含量和体内磷酸酶(碱性磷酸酶和酸性磷酸酶)活性的慢性(30 d)影响.结果显示:(1)Cu~(2+)和Zn~(2+)能明显降低糠虾存活率,金属离子浓度增高,存活率降低,其中Cu~(2+)引起的存活率降低更为明显,在最高浓度(80 μg/ L)Cu~(2+)组中糠虾的存活率仅为同浓度Zn~(2+)组的1/3,两组均明显低于对照组(P<0.05);Cu~(2+)和Zn~(2+)能明显降低糠虾蜕皮次数,最高浓度(80 μg/ L)Cu~(2+)和Zn~(2+)中糠虾的蜕皮数分别为11.00±1.73(次)和11.33±0.58(次),分别约为对照组的55.92%和57.6%.(2)Cu~(2+)和Zn~(2+)均能明显影响糠虾蛋白含量,其中Cu~(2+)的影响力更强,最高浓度(80 μg/ L)Cu~(2+)组糠虾体内蛋白白含量占湿重比为9.78%,仅约为对照组的1/2.(3)Cu~(2+)和Zn~(2+)能明显抑制糠虾体内两种磷酸酶的活力,碱性磷酸酶和酸性磷酸酶活力能作为糠虾监测环境重金属污染(Cu~(2+)和Zn~(2+))的检测指标.

Effects of Cu~(2+) and Zn~(2+)on growth and activity of phosphatase of Neomysis japonica Nakazawa

The effects of different concentration Cu~(2+) and Zn~(2+) (10,20,40 and 80 μg/L)on the growth, the protein content and the activity of phosphatase of the Neomysis japonica(Nakazawa)were studied by feeding experiment in aquarium of Laboratory for 30 d.The results showed as follows:(1) The survival of mysids was obviously lower in Cu~(2+) and Zn~(2+) groups than in control group(P<0.05),especially in Cu~(2+) group.The survival in most highest concentration Cu~(2+) group (80 μg/L) was only 1/3 in same concentration Zn~(2+) group;Compared with the control group, molt number of mysids in Cu~(2+) and Zn~(2+) groups obviously decreased(P<0.05).(3) The activities of phosphatase (Alkaline phosphatase and Aaciphosphatase) were decreased after Cu~(2+) and Zn~(2+) treated.The activities of phosphatase in mysids could be used to monitor Cu~(2+) and Zn~(2+) pollution.