含硝氮废水的好氧反硝化处理及其系统微生物群落动态分析

利用好氧反硝化细菌强化生物陶粒反应器处理含硝氮废水,探讨了生物陶粒反应器中好氧反硝化生物脱氮的实现过程,并运用变性梯度凝胶电泳技术(DGGE)对微生物菌群结构稳定性进行了分析.结果表明,在水力负荷0.75 m/h,气水比5∶1～10∶1,水温15～23℃,进水COD负荷1.92～5.98　kg/(m³·d),硝氮负荷0.60～1.34 kg/(m³·d)的条件下,生物陶粒反应器在稳定运行阶段可以基本实现对硝酸氮的完全去除,对总氮的去除率最高可达95.73％,出水中亚硝酸盐一直保持在较低水平.PCR-DGGE指纹图谱显示,微生物多样性与废水的处理效果出现协同变化的特征.在整个运行阶段,好氧反硝化菌群X31在反应器中稳定存在,并始终是优势菌群.

Aerobic Denitrification of Nitrate Wastewater and Changes of Microbial Community Structure in a Bio-ceramic Reactor

Biological treatment of nitrate wastewater with aerobic denitrifiers in a bio-ceramic reactor was investigated. And denaturing gradient gel electrophoresis (DGGE) method was used for analyzing the stability of microbial community structure. At the condition of the influent with hydraulic loading 0.75 m/h, gas/water ratio 5:1-10:1, temperature 15-23 degrees C, COD loading rate 1.92-5.98 kg/(m3 x d) and NO3- -N loading rate 0.60-1.34 kg/(m3 x d), high nitrate nitrogen removal of almost 100% and the maximum total nitrogen removal of up to 95.73% were achieved during stable operation stage. In addition, the nitrite concentration of effluent was lower. The PCR-DGGE profiles showed that shift of microbial diversity corresponded to the effect of nitrate removal and the preponderant populations for aerobic denitrification were steady during the test period.