厌氧氨氧化菌混培物保藏方法的研究

厌氧条件下,采用了4℃以(NH4)2SO4为保藏基质、自然环境温度以(NH4)2SO4为保藏基质、4℃用NH4Cl代替(NH4)2SO4作为保藏基质3种方法保藏厌氧氨氧化菌混培物.结果表明,4℃以(NH4)2SO4为保藏基质的方法保藏厌氧氨氧化菌混培物最有效,但自然环境温度以(NH4)2SO4为保藏基质的方法保藏厌氧氨氧化菌混培物最经济;在保藏试验的5个月内,3种保藏方法下的厌氧氨氧化活性均先快速下降后趋于稳定,趋于稳定时的活性保留率分别为75.3%,66.3%和47.3%;恶劣的生存环境下,胞外多聚物不仅可维持厌氧氨氧化菌混培物颗粒结构的稳定性,还可使菌群聚集成团从而增大菌群密度以减少代谢过程中的能量损失;无外界颜色干扰时,厌氧氨氧化菌混培物颜色由血红素c含量决定,且与活性直接相关.

Preservation of ANAMMOX bacteria

Under anaerobic conditions, three methods on preservation of ANAMMOX bacteria were studied, which were at 4℃ with (NH4)2SO4 as storage matrix,at natural temperature with (NH4)2SO4 and at 4℃ with NH4Cl, respectively. At 4℃ with (NH4)2SO4was the most effective method, but at ambient temperature with (NH4)2SO4was the most economical method. During the 5-month preservations, ANAMMOX activities with the three methods decayed rapidly at the beginning, and stabilized gradually later on. When activities gone steady, the survival activities with the three methods were 75.3%, 66.3% and 47.3%, respectively. Extracellular polymers not only maintained the structural integrity of ANAMMOX granules, but also increased the cell density for decreasing the energy loss and the injury of starvation in severe environment. Without color interference, the color of ANAMMOX bacteria was directly correlated with the content of heme c, which could reflect the activities of ANAMMOX bacteria.