| 两种假单孢菌中二氯酚降解酶活性及其定域研究 |
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| 引用本文： | 戴树桂,庄源益,陈勇生,陈广宏,陈丽侠,肖钧.两种假单孢菌中二氯酚降解酶活性及其定域研究[J].环境科学学报,1996,16(2):173-178. |
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| 作者姓名： | 戴树桂 庄源益 陈勇生 陈广宏 陈丽侠 肖钧 |
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| 作者单位： | 南开大学环境科学系 |
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| 基金项目： | 高等学校博士学科点专项基金 |
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| 摘 要： | 对两种有降解二氯酚能力的假单孢菌Ｐｓｅｕｄｏｍｏｎａｓ ｓｐ．ＤＣＰ－１和Ｐｓｅｕｄｏｍｏｎａｓ ｓｐ．ＤＣＰ－２中二氯酚降解酶活性及定域进行了测定，结果表明该酶的活力与菌种、培养等因素有关，与诱导物的关系不显；酶主要定域在膜周及膜内；酶的活力在两种菌中的水平相当；酶的比活力在ＤＣＰ－１的膜周处表现出相当高的水平。
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| 关 键 词： | 二氯酚 降解酶 比活力 假单胞菌 活力 |
| 收稿时间： | 1994/8/22 0:00:00 |
STUDY ON ENZYME ACTIVITY AND DISTRIBUTION IN TWO STRAINS OF BACTERIA BY DICHLOROPHENOL DEGRADATION |
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| Dai Shugui,Zhuang Yuanyi,Chen Yongsheng,Chen Guanghong,Chen Lixia and Xiao Jun.STUDY ON ENZYME ACTIVITY AND DISTRIBUTION IN TWO STRAINS OF BACTERIA BY DICHLOROPHENOL DEGRADATION[J].Acta Scientiae Circumstantiae,1996,16(2):173-178. |
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| Authors: | Dai Shugui Zhuang Yuanyi Chen Yongsheng Chen Guanghong Chen Lixia and Xiao Jun |
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| Affiliation: | Department of Environmental Sciences, Nankai University, Tianjin 300071,Department of Environmental Sciences, Nankai University, Tianjin 300071,Department of Environmental Sciences, Nankai University, Tianjin 300071,Department of Environmental Sciences, Nankai University, Tianjin 300071,Department of Environmental Sciences, Nankai University, Tianjin 300071 and Department of Environmental Sciences, Nankai University, Tianjin 300071 |
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| Abstract: | Activity and distribution of enzyme produced by two strains of bacteria (Psedomonas sp.DCP-1 and Psedomonas sp. DCP-2) , which can utilize dichlorophenol, have been measured.The results shown that enzyme activity is effected by bacteria and culture time, and not by inducer; the distribution of enzyme produced by the two strains of bacteria is mainly in piroplasmic and intercellular; the specific activity in piroplasmic of Pseudomonas, sp. DCP-1 is higher than that in DCP-2. |
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| Keywords: | dichlorophenol degrading-enzyme specific activity distribution Pseudomonas sp DCP-1 Pseudomonas sp DCP-2 |
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