Ni2＋对活性污泥活性及群落多样性的影响

通过检测活性污泥的电子传递体系活性以及生物多样性，研究Ni2＋对活性污泥微生物活性及群落多样性的影响。结果表明：与对照系统相比，5mg／L的Ni2＋对2，3，5-lriphenylteItrazoliumchloride（TTC．ETS）活性未产生显著的影响；但当Ni2＋的浓度进一步增大到10、20和40mg／L后，其对序批式反应器内活性污泥TTC—ETS活性的抑制率分别达到（36．794-11．14）％、（55．88±13．90）％和（70．97±6．78）％。低浓度Ni2＋．能增强活性污泥微生物对碳源的利用，但高于10mg／L的Ni2＋则显著抑制了活性污泥微生物对碳源的利用。各个SBR系统中微生物群落最常见的物种相近，物种丰富度和均一性则均有所不同，其中群落物种丰富度随着Ni2＋浓度的增加而逐渐减小。TTC—ETS活性、平均每孔颜色变化率、Shan—liOn指数和Simpson指数，与Ni2＋的胁迫浓度之间的显著相关性表明，它们均可有效地表征Ni2＋胁迫对活性污泥微生物活性及群落多样性的影响程度。

Effects of Ni（ lI ）on activity and community diversity of activated sludge

The effects of Ni（II） on the activity and community diversity of activated sludge were investiga- ted by determining the electron transport system （ETS） activity and microbial metabolic diversity （Biolog）. The results show that no significant inhibition could be detected in the 2, 3, 5-triphenyheltrazolium chloride （TTC- ETS） values between the control system （CK） and the sequencing batch reactor （SBR） system loaded with 5 mg/L Ni（II）. However, as compared to the CK, the inhibitory rates are （36.79 ＋__ 11.14）%, （55.88 ＋ 13.90） % , and （70. 97 _＋6. 78）% for the TTC-ETS of sludge microorganisms in SBR systems fed with 10, 20, and 40 mg/L Ni（II） , respectively. The carbon utilization capacity of the sludge microorganisms could be im- proved by feeding low concentration of Ni（II） , while the carbon utilization capacity could be significantly inhibi- ted as the feeding concentration of Ni（lI） increasing up to 10 mg/L. According to the diversity index analysis, the microbial communities in the five SBR systems have a resemblance in the most common species, while differ- ences in the richness and homogeneity. The Ni（I） feeding concentrations significantly correlate to the TTC- ETS, the average well colour development （AWCD）, the Shannon index, and the Simpson index, suggesting that these indexes could effectively predict the effects of Ni（II） on the activity and community diversity of activa- ted sludge.