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亮菌多糖提取中脱蛋白和脱色方法比较
引用本文:黄纯,马驰,宋慧智,李甜甜.亮菌多糖提取中脱蛋白和脱色方法比较[J].药学与临床研究,2007,15(1):45-46.
作者姓名:黄纯  马驰  宋慧智  李甜甜
作者单位:中国药科大学高等职业技术学院,镇江,212003
基金项目:中国药科大学校青年教师科技基金(编号:D0510)
摘    要:目的:探讨亮菌多糖提取过程中的脱蛋白和脱色方法.方法:以蛋白质脱除率和多糖保留率为考察指标,比较Sevage法、氯化钠法、氯化钙法、三氯乙酸法及盐酸法脱蛋白效果.以色素脱除率作为考察指标,比较过氧化氢和活性炭的脱色效果.结果:Sevage法脱蛋白效果较好且多糖得率最高;过氧化氢的脱色效果优于活性炭.结论:确定Sevage法和过氧化氢法为亮菌多糖提取的脱蛋白和脱色的方法.

关 键 词:脱蛋白  脱色  亮菌多糖  亮菌多糖  多糖提取  脱蛋白  脱色方法  比较  polysaccharide  extraction  deproteinization  过氧化氢法  多糖得率  结果  脱色效果  活性炭  色素  盐酸法  三氯乙酸法  氯化钙法  钠法  指标  考察
文章编号:1673-7806(2007)01-45-02
收稿时间:2006-08-18
修稿时间:2006年8月18日

Study on deproteinization and decoloration in extraction of Armillariella polysaccharide
HUANG Chun,MA Chi,SONG Hui-zhi,LI Tian-tian.Study on deproteinization and decoloration in extraction of Armillariella polysaccharide[J].Pharmacertical and Clinical Research,2007,15(1):45-46.
Authors:HUANG Chun  MA Chi  SONG Hui-zhi  LI Tian-tian
Abstract:Objective: To investigate the method on deproteinization and decoloration in extraction of Armillariella polysaccharide.Methods: The effect of deproteinization with different methods(such as Sevage,Sodium chloride,Calcium chloride,TCA and Hydrochloric acid)were evaluated in terms of the ratio of protein removing and polysaccharide holding,and the decoloration effect of hydrogen peroxide(H2O2) or activated carbon was also calculated.Results: The deproteinization effect was optimal with Sevage method and H2O2 also achieve a high decoloration rate.Conclusion: Sevage method and H2O2 could be used for extraction of Armillariella polysaccharide.
Keywords:Deproteinization  Decoloration  Armillariella polysaccharide(ALP)
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