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硫化物微生物传感器的研究
引用本文:白志辉,王晓辉,罗湘南,孙裕生.硫化物微生物传感器的研究[J].河北科技大学学报,1999,20(1):10-13.
作者姓名:白志辉  王晓辉  罗湘南  孙裕生
作者单位:河北科技大学环境科学与工程系,石家庄,050018
摘    要:从硫铁矿的酸性土壤中分离、筛选出氧化硫硫杆菌,将其固定化制备微生物膜,再与氧电极组装成微生物传感器,用于样品中微量硫化物的测定。实验研究表明:该传感器响应S2-浓度线性范围为0.06~1.50mg/L;响应时间为3~6min;30d内测定500余次,灵敏度保持不变;取煤气站脱硫塔入口和出口煤气样品进行测定,与亚甲蓝比色法测定结果一致;取合成水样品进行回收率实验,结果为93.8%~105.0%

关 键 词:微生物传感器  微生物电极  硫化物的测定
修稿时间:1998-11-03

Study on the Microbial Sensor for Sulfide
Bai Zhihui,Wang Xiaohui,Luo Xiangnan,Sun Yusheng.Study on the Microbial Sensor for Sulfide[J].Journal of Hebei University of Science and Technology,1999,20(1):10-13.
Authors:Bai Zhihui  Wang Xiaohui  Luo Xiangnan  Sun Yusheng
Abstract:Thiobacillus thioxidans was separated from acid soil.The microbial membrane,prepared by immobilized microorganisms,was attached to the surface of oxygen electrode to form microbial sensor for the determination of sulfide.The liner response range of the sensor is 0.06~1.50 mg/L and the response time is 3~6 min for sulfide.The sensitivity of the sensor was constant in 30 days and more than 500 assays.The microbial sensor was applied to the determination of gas samples.A good agreement was obtained between the microbial sensor and the methylene blue method.The recovery rates for sulfide added into water were 93.8%~105.0%.
Keywords:microbial  sensor  microbial  electrode  determination  of  sulfide  
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