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厌氧氨氧化菌混培物生长及代谢动力学研究
引用本文:郑平,胡宝兰.厌氧氨氧化菌混培物生长及代谢动力学研究[J].生物工程学报,2001,17(2):193-198.
作者姓名:郑平  胡宝兰
作者单位:浙江大学环境工程系
基金项目:国家自然科学基金资助项目(30070017).
摘    要:研究了厌氧氨氧化菌混培物的动力学特性.测得细胞产率系数1.573mgVS(mmolNH4+)-1;细胞衰减常数0.052mgVS(g@VS@d)-1.厌氧氨氧化菌混培物的最大氨氧化速率1.320~2.761mmol(gVS@d)-1,最大亚硝酸盐转化(反硝化)速率14.497mmol(gVS@d)-1.厌氧氨氧化菌混培物利用氨的Km值1.801~4.215mmol@L-1,利用亚硝酸盐的Km值0.468mmol@L-1.氨自身的抑制常数38.018~98.465mmol@L,实际最大氨氧化速率的氨浓度16.656mmol@L-1.亚硝酸盐对厌氧氨氧化的抑制常数5.401~11.995mmol@L-1.厌氧氨氧化的最适pH7.605.厌氧氨氧化的最适温度30℃.Vmaxa、Kma、Kia和Kin的活化能依次为37.316、30.239、33.695和30.473kJ@mol-1.

关 键 词:厌氧氨氧化菌  动力  硝化  反硝化  混培物  生长  代谢动力学
文章编号:1000-3061(2001)02-0193-06
修稿时间:2000年7月26日

Kinetics of Anaerobic Ammonia Oxidation
ZHENG Ping,\ HU Bao\|Lan.Kinetics of Anaerobic Ammonia Oxidation[J].Chinese Journal of Biotechnology,2001,17(2):193-198.
Authors:ZHENG Ping  \ HU Bao\|Lan
Affiliation:Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China.
Abstract:The kinetic characteristics of anaerobic ammonia oxidation was studied. The cell yield was 1.573 mgVS (mmolNH4+)-1 and the cell decay coefficient was 0.052 mgVS(gVS.d)-1. The maximal rate of anaerobic ammonia oxidation and nitrite conversion rate by the mixed culture were 1.320-2.761 mmol(gVS.d)-1 and 14.497 mmol(gVS.d)-1, respectively. Km for ammonia and for nitrite were 1.801-4.215 and 0.468 mmol.L-1. Ki for ammonia was 38.018-98.465 mmol.L-1, the optimal ammonia concentration was 16.656 mmol.L-1. Ki for nitrite was 5.401-11.995 mmol.L-1. The optimal pH was around 7.5 and the theoretical value was 7.605. The optimal temperature was about 30 degrees C. The activation energy for Vmaxa, Kma, Kia and Kin was 37.316, 30.239, 33.695 and 30.473 kJ.mol-1.
Keywords:anaerobic ammonia oxidation  kinetics  nitrification  denitrification
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