高效液相色谱法测定青贮饲料桑中的乳酸、乙酸和丙酸含量条件探讨

本文应用高效液相色谱法（HPLC）对青贮饲料桑中的乳酸、乙酸和丙酸含量测定进行了研究。通过对色谱柱、流动相、流速及样品处理条件进行优化，建立了一种应用HPLC法同时测定青贮饲料桑中乳酸、乙酸和丙酸含量的方法。研究结果表明：乳酸、乙酸和丙酸在一定浓度下具有良好的线性关系，且相关系数R2均大于0.999，加标回收率为98.35% ~ 104.24%，标准品中回收率和精密度试验相对标准偏差（RSD）为0.01%~0.58%，表明该方法准确性较好。3种物质检出限为4.928 ~ 9.489 mg/L，适用于青贮饲料桑中有机酸的定量检测。 关键词] 高效液相色谱（HPLC）|青贮饲料桑|乳酸|乙酸|丙酸

Determination of lactic acid,acetic acid and propionic acid contentin mulberry silage by high performance liquid chromatography

In this paper,the determination of lactic acid,acetic acid and propionic acid in mulberry silage was studied by high performance liquid chromatography(HPLC).By optimizing the chromatography column,mobile phase,flow rate and extraction conditions,the method for simultaneous determination of lactic acid,acetic acid and propionic acid in mulberry silage by HPLC method was established.The results showed that lactic acid,acetic acid and propionic acid had a good linear relationship at a certain concentration,and the correlation coefficient R² was greater than 0.999.The recovery rate of the three acids was 98.35%~104.24%.The relative standaral deviation(RSD)of the recovery rate and accuracy tests was 0.01%~0.58%.This indicated that the method has good accuracy.The detection limit of the three acids was 4.928~9.489 mg/L.The method could be used to the quantitative detection of organic acids in mulberry silage.