植物次生物质对棉铃虫谷胱甘肽S-转移酶和乙酰胆碱酯酶的影响

分别用含０．０１％的芸香甙，槲皮素２－十三烷酮的人工饲料连续饲养棉铃虫Ｈｅｌｉ－ｃｏｖｅｒｐａａｒｍｉｇｅｒａＨｕｂｎｅｒ１～７代，测定不同处理的棉铃虫种各谷胱甘肽Ｓ－转移酶（ＧＳＴ）和乙酰胆碱酯酶（ＡＣｈＥ）的活性变化以及幼虫对杀虫剂的反应，以单独人工饲料养的各种作对照，芸香甙Ｆ１代对甲基硫磷的耐药性提高３倍，槲皮素Ｆ１，Ｆ２代和２－十三烷酮Ｆ１代对甲基对硫磷的耐药性有所提高，对灭多威和溴氰菊酯

EFFECTS OF PLANT ALLELOCHEMICALS ON THE GLUTATHIONE-S-TRANSFERASE AND ACETYLCHOLINESTERASE IN HELICOVERPA ARMIGERA

Effects of plant allelochemicals on glutathione-S-transferase (GST) and acetylcholinesterase (AChE) activities were studied with midgut or head homogenates of larvae in Helicoverpa armigera Hubner that had reared on an artificial diet containing 0. 01% of rutin, quercetin or 2-tridaconone for 1 - 7 generations. The toxicity of methyl parathion to rutin-fed F1 , F3 and F4 generation was 3 times, 2 times and 2 times lower comparing with artificial diet-fed generations respectively. The activity of GST from cotton bollworm fed on the artificial diet containing allelochemicals is much higher than those fed on artificial diet. The affinity between AChE to acectylthiocholine (ATCh) and the sensitivity of AChE to paraoxon increased significantly. The effect of plant allelochemicals on cotton bollworm susceptibility to insecticides may be determined by the integrated results of the detoxifying enzymes and AChE changes.