二斑叶螨对梅岭霉素和溴虫腈的抗性选育及其解毒酶活力变化

在温室内模拟田间药剂的选择压力,用梅岭霉素和溴虫腈对二斑叶螨Tetranychus urticae以浸渍法逐代处理,选育其抗性种群,评价其抗性风险性.结果表明,选育至12代,对梅岭霉素抗性增长到9.2倍,对溴虫腈抗性增长到10.0倍.酶抑制剂和离体酶活性的测定结果表明,二斑叶螨对梅岭霉素的抗性可能与羧酸酯酶、多功能氧化酶解毒作用的增强和药剂对害螨体壁穿透性降低有关;而药剂对害螨体壁穿透性降低和谷胱甘肽-S-转移酶及酯酶代谢作用的增强可能是二斑叶螨对溴虫腈产生抗性的主要原因.

Resistance selection by meilingmycin and chlorfenapyr and activity changes of detoxicated enzymes in Tetranychus urticae

In order to evaluate the resistance risk of meilingmycin and chlorfenapyr to Tetranychus urticae, resistance selection by slide slip method were carried out in laboratory. The related susceptible population of T. urticae was separately treated with meilingmycin and chlorfenapyr simulating field selection pressure in order to breeding its resistance. The results showed that the resistance of T. urticae had reached 9.2 folds to meilingmycin and 10 folds to chlorfenapyr after 12 generations treatment. The resistance mechanism was studied by detecting the activity changes of enzymic inhibitors and detoxicated enzymes. The rise of resistance to meilingmycin was because of the increased activities of carboxy lesterase, microsomal oxidase and decreased cuticular penetration. The resistance to chlorfenapyr was attributed to increased activities of glutathion-S-transferase and decreased cuticular penetration.