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菜心内生细菌CX-PA的鉴定及生防作用
引用本文:李静,陈维信,刘爱媛,冯淑杰,肖晶.菜心内生细菌CX-PA的鉴定及生防作用[J].中国生物防治,2007,23(4):362-367.
作者姓名:李静  陈维信  刘爱媛  冯淑杰  肖晶
作者单位:华南农业大学园艺系,广州,510642
摘    要:从菜心植株内分离到一株具有生防活性的细菌CX-PA,通过对其形态特征和生理生化特性测定,16S rDNA部分序列同源性分析,鉴定为多粘类芽孢杆菌。采用浸种、喷雾接种处理,CX-PA可进入小白菜、大白菜和菜心体内定殖。培养基平板对峙测定,CX-PA对菜心炭疽病菌等7种病原菌有较强的拮抗作用。盆栽防治试验表明,CX-PA对菜心炭疽病和霜霉病的防治效果分别为70.8%和64.8%。

关 键 词:菜心  多粘类芽孢杆菌  生物防治
文章编号:1005-9261(2007)04-0362-06
修稿时间:2007-02-12

Identification and Biocontrol Effect of Bacterial Strain CX-PA Selected from Flowering Chinese Cabbage
LI Jing,CHEN Wei-xin,LIU Ai-yuan,FENG Shu-jie,XIAO Jing.Identification and Biocontrol Effect of Bacterial Strain CX-PA Selected from Flowering Chinese Cabbage[J].Chinese Journal of Biological Control,2007,23(4):362-367.
Authors:LI Jing  CHEN Wei-xin  LIU Ai-yuan  FENG Shu-jie  XIAO Jing
Affiliation:College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Abstract:Bacterial strain CX-PA is a biocontrol agent isolated from flowering Chinese cabbage.It was identified as Paenibacillus polymyxa according to the characteristics of its morphological,physiological, biochemical characters and the homology analysis of the partial sequence of 16S rDNA.By seed soaking and leaf spraying,the strain CX-PA could colonize in cabbage,celery cabbage and flowering Chinese cabbage.In the dual-culture test in plates, the strain showed strong inhibitory to Colletotrichum higgisianum,C.musae,Phytophthora capsici,Peronophythora litchi,Alternaria mali,Fusarium oxysporum and Penicillium digitatum.In the pot experiment,strain CX-PA had 70.8% and 64.8% control effect on flowering Chinese cabbage anthracnose disease and flowering Chinese cabbage downy mildew,respectively.
Keywords:flowering Chinese cabbage  Paenibacillus polymyxa  biological control
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