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| 中黑盲蝽对四季豆豆荚和棉铃虫卵的取食选择性 | |
| 引用本文： | 李文静,苑鹤,陆宴辉,李强,吴孔明.中黑盲蝽对四季豆豆荚和棉铃虫卵的取食选择性[J].植物保护,2015,41(4):29-34. |
| 作者姓名： | 李文静 苑鹤 陆宴辉 李强 吴孔明 |
| 作者单位： | 1. 东北农业大学生命科学学院，哈尔滨 150030; 中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京 100193 2. 中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京 100193 3. 东北农业大学生命科学学院,哈尔滨,150030 |
| 基金项目： | 国家自然科学基金（31272115） |
| 摘 要： | 盲蝽类害虫具有杂食性特点,但已有的取食行为研究主要集中于植食性,缺乏对其食性的全面认识。本文观察了中黑盲蝽在有选择和无选择条件下对四季豆豆荚和棉铃虫卵的取食与选择行为,并利用刺探电位技术分析了其对四季豆豆荚和棉铃虫卵的刺吸行为。研究显示:3种处理上中黑盲蝽总取食时间比例分别为:混合食物(四季豆豆荚和棉铃虫卵)38%、四季豆豆荚33%、棉铃虫卵27%,混合食物上取食四季豆豆荚的时间比例(22%)高于棉铃虫卵(16%)。6h测试时间内,中黑盲蝽刺吸四季豆豆荚时间为6 595s,高于棉铃虫卵(3 641s);单次刺吸时间以在四季豆豆荚上较高(334s)。以上结果表明中黑盲蝽喜欢取食混合食物,且倾向取食刺吸植物。研究结果对进一步解析中黑盲蝽的杂食性,探明其在生态系统中的地位及开发人工饲料有重要科学意义。 |
| 关 键 词： | Cry1Ac 蛋白 棉铃虫 APN1 原核表达 结合位点 |
| 收稿时间： | 4/4/2014 12:00:00 AM |
| 修稿时间： | 2014/6/22 0:00:00 |
Diet selection of Adelphocoris suturalis on Phaseolus vulgaris pods and Helicoverpa armigera eggs |
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| Li Wenjing,Yuan He,Lu Yanhui,Li Qiang,Wu Kongming.Diet selection of Adelphocoris suturalis on Phaseolus vulgaris pods and Helicoverpa armigera eggs[J].Plant Protection,2015,41(4):29-34. | |
| Authors: | Li Wenjing Yuan He Lu Yanhui Li Qiang Wu Kongming |
| Affiliation: | 1. College of Life Sciences, Northeast Agricultural University, Harbin 150030, China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
| Abstract: | Binding assay between Cry1Ac toxin and BBMVs from Helicoverpa armigera was analyzed in this study. The full length gene and the fragments of apn1 were cloned and expressed in Escherichia coli Rosetta (DE3) strain. The binding between full length/fragments of APN1 expressed in E. coli and Cry1Ac toxin was performed by Ligand blotting. The results showed that BBMVs from H. armigera could bind Cry1Ac toxin; the full length APN1 and APN1 fragments H1 (33-257), H2 (258-547), and H3 (548-798) expressed in prokaryotic system could bind Cry1Ac toxin. It was the first report that H3 (548-798) could bind with Cry1Ac. The results may provide a new clue for functional studies on other Bt toxin receptors in vitro |
| Keywords: | Cry1Ac toxin Helicoverpa armigera aminopeptidase N1 prokaryotic expression binding site |
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