栉孔扇贝Dmrt1的分子鉴定及表达模式分析

DMRT1是DMRT家族重要成员之一，主要参与动物性别决定和分化调控，但在不同动物中其表达和功能调控作用存在差异。本研究采用生物信息学方法分析了栉孔扇贝（Chlamys farreri）Dmrt1的序列特征，采用半定量RT-PCR技术确定了其mRNA在成体性腺、闭壳肌、外套膜、鳃和肾等组织中的分布，定量RT-PCR和原位杂交技术揭示了Dmrt1 mRNA在性腺中的时空表达特征。结果显示：栉孔扇贝Dmrt1序列中含有DMRT家族保守的DM结构域；其mRNA仅在栉孔扇贝性腺中表达，在精巢中的表达明显高于卵巢，并且以生长期精巢的表达水平最高；原位杂交检测Dmrt1阳性信号主要定位于生殖细胞的细胞质中。研究结果表明，Dmrt1在栉孔扇贝性腺中的表达特征与大部分动物性腺中的表达特征基本一致，推测其可能参与性别分化和精巢发育的功能调节。

Molecular identification and expression pattern analysis of Dmrt1 in the scallop Chlamys farreri

DMRT1, an important member of the DMRT family, is mainly involved in sex determination and sex differentiation regulation in animals, but its expression characteristics and function are different in different species. In the present study, we analyzed Chlamys farreri Dmrt1 sequence characteristics using bioinformatics methods; determined Dmrt1 mRNA tissue distribution in adult gonad, adductor muscle, mantle, gill, and kidney tissue using semi-quantitative RT-PCR; and revealed its spatiotemporal expression pattern in C. farreri gonads using quantitative RT-PCR and in situ hybridization. The results showed that the deduced amino acid sequence of DMRT1 contained the conserved DM domain of the DMRT family; its mRNA was expressed exclusively in the gonads, and the mRNA levels in the testes were significantly higher than those in the ovaries, and it was the highest in the testis at the growing stage. Moreover, Dmrt1 positive signals were located in C. farreri germ cell cytoplasm. We suggested that Dmrt1 expression characteristics in C. farreri adult gonads is consistent with that in most animals, and may be involved in regulating C. farreri sex differentiation and testis development.