1994年浙江省对虾暴发性流行病病原及传播途径的初步调查

用Ｔ—Ｅ染色、单克隆抗体的ＥＬＩＳＡ现场诊断、组织切片和Ｈ—Ｅ染色和电镜观察等不同方法对１９９４年浙江省乐清和温岭两地区虾池及海区样品进行了现场和实验室检查。结果说明，１９９４年浙江省对虾暴发性流行病实质上是杆状病毒性的皮下及造血组织坏死，病原为ＨＨＮＢＶ。检查到的ＨＰＶ不是这场暴发病的病原。对非对虾类生物和对虾鲜活饲料的单抗ＥＬＩＳＡ检测表明，海区张网饲料可能是１９９４年浙江对虾暴发性流行病的主要传染源，病虾池中除对虾外，其它甲壳类生物在该病的传播上可能也起到一定的作用。这些生物除了可能传播ＨＨＮＢＶ外，还可能传播ＨＰＶ。

PRIMARY INVESTIGATION ON THE VIRAL PATHOGENS AND TRANSMISSION ROUTE DURING THE EXPLOSIVE EPIDEMIC SHRIMP DISEASE IN ZHEJIANG IN 1994

Several different diagnostic methods, such as T-E staining,ELISA,histological section and electron microscopy were applied to detect the samples collected from shrimp ponds and coast area in Yueqing and Wenting in Zhejiang province in 1994. The results showed that the causative pathogen for the explosive epidemic disease of shrimp (EEDS) in Zhejiang province in 1994 was HHNBV instead of HPV even though HPV was detectable. ELISA tests for non-penaeid animals and fresh feed suggested that the chief infective sources of EEDS in Zhejiang might be fresh feed netted from coasta area.Other crustacean,besides infected shrimp,might spread HHNBV and HPV as well.