恶喹酸与诺氟沙星对异育银鲫体内嗜水气单胞菌的抑菌效果比较

在水温(25±2)℃下,给体质量150~200g的异育银鲫分别口灌3种剂量(20、30、40mg/kg)的诺氟沙星和恶喹酸,结合这两种药物对嗜水气单胞菌AH10的体外药效学研究和对异育银鲫单次口灌不同剂量的诺氟沙星、恶喹酸的体内药代动力学,研究诺氟沙星、恶喹酸对异育银鲫体内嗜水气单胞菌AH10的抑菌效果。试验结果表明,恶喹酸和诺氟沙星对嗜水气单胞菌AH10的最小抑菌质量浓度分别为1μg/mL和0.5μg/mL。口灌上述3种剂量恶喹酸、诺氟沙星后,异育银鲫血浆中恶喹酸的最大药物质量浓度分别为4.1μg/mL、6.0μg/mL和8.89μg/mL;诺氟沙星的最大药物质量浓度分别为11.5μg/mL、15.1μg/mL和18.9μg/mL;恶喹酸的最大药物质量浓度/最小抑菌质量浓度分别为4.1、6.0和8.89;诺氟沙星的最大药物质量浓度/最小抑菌质量浓度分别为23、30.2和37.8;恶喹酸0~24h内药—时曲线下面积/最小抑菌质量浓度分别为21.6214、33.1449、39.1846;诺氟沙星0~24h内药—时曲线下面积/最小抑菌质量浓度分别为274.75、451.55、578.35。综合0~24h内药—时曲线下面积/最小抑菌质量浓度、最大药物质量浓度/最小抑菌质量浓度这两个指标可知,诺氟沙星对异育银鲫体内的嗜水气单胞菌AH10抑制效果强于恶喹酸。

Comparative Bacteriostasis of Oxolinic Acid and Norfloxcin against Pathogenic Bacterium Aeromonas hydrophila in Allogyogenetics Silver Crucian Carp Carassius auratus gobelio

In vitro and in vivo pharmacokinetics of oxolinic acid and norfloxcin against pathogenic bacteri‐um Aeromonas hydrophila (AH10) were studied in allogyogenetics silver crucian carp Carassius auratus gobelio with body weight of 150—200 g singly and orally administrated at a dose of 20 ,30 ,and 40 mg per kg body weight at water temperature of (25 ± 2) ℃ .The minimal inhibitory concentration (MIC) on AH10 was shown to be 1μg/mL in oxolinic acid and 0 .5μg/mL in norfloxcin .The maximum serum concentration of oxolinic acid in allogyogenetics silver crucian carp was 4 .1 μg/mL at a dose of 20 mg per kg body weight ,6 .0 μg/mL at a dose of 30 mg per kg body weight ,and 8 .89μg/mL at a dose of 40 mg per kg body weight ,and the maximum serum concentration of norfloxcin were 11 .5 μg/mL at a dose of 20 mg per kg body weight ,15 .1 μg/mL at a dose of 30 mg per kg body weight ,and 18 .9 μg/mL at a dose of 40 mg per kg body weight .Cmax of oxolinic acid /MIC of oxolinic acid was 4 .1 ,6 .0 and 8 .89 at various doses ,respec‐tively ;Cmax of norfloxcin /MIC of norfloxcin was 23 ,30 .2 and 37 .8 at various doses ,respectively .The ar‐ea under the curve (AUC24 ,0—24 h) of oxolinic acid/MIC of oxolinic acid was 21 .6214 ,33 .1449 and 39 .1846 at various doses ,respectively ;AUC24 of norfloxcin /MIC of norfloxcin was 274 .75 ,451 .55 and 578 .35 at various doses ,respectively .Based on the AUC24/MIC and Cmax/MIC ,there was greater bacteri‐ostasis in norfloxcin than in oxolinic acid against A .hydrophila in allogyogenetics silver crucian carp .